FlyBase Allele Report: Dmel\Decay[GD8484]

General Information

Symbol

Dmel\Decay^GD8484

Species

D. melanogaster

Name

FlyBase ID

FBal0209339

Feature type

allele

Associated gene

Dmel\Decay

Associated Insertion(s)

Carried in Construct

P{GD8484}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8484}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8484

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^C10

(Shinoda et al., 2019)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible | adult stage, with Scer\GAL4^ap-md544

(Shinoda et al., 2019)

visible | adult stage, with Scer\GAL4^C10

(Shinoda et al., 2019)

Phenotype Manifest In

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^ap-md544

(Shinoda et al., 2019)

wing, with Scer\GAL4^C10

(Shinoda et al., 2019)

Detailed Description

Statement

Reference

The expression of Decay^GD8484 under the control of Scer\GAL4^ap-md544, with or without Dicer-2 expression (for a more efficient RNAI) leads to curly but not opaque wings, which indicates growth inhibition during development without apoptosis.

Expression under the control of Scer\GAL4^C10 leads to a significant decrease in wing size, as compared to controls. Wing discs do not show changes in apoptosis (assessed by PARP:VENUS signal and TUNEL assay), as compared to controls.

(Shinoda et al., 2019)

Adults expressing Decay^GD8484 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Pupae expressing decay^GD8484 under the control of Scer\GAL4^NP1 undergo midgut histolysis at a similar rate as wild-type animals.

(Denton et al., 2009)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 10-20% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8484

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Decay^GD8484, Scer\GAL4^NP0001 is a non-enhancer of embryonic/larval midgut | P-stage phenotype of Atg18a^GD12342, Scer\GAL4^NP0001

(Denton et al., 2009)

Decay^GD8484, Scer\GAL4^NP0001 is a non-enhancer of embryonic/larval midgut | P-stage phenotype of Atg1^GD7149, Scer\GAL4^NP0001

(Denton et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Pupae expressing decay^GD8484 under the control of Scer\GAL4^NP1 in a Ice^Δ1 Nc^unspecified background undergo midgut histolysis at a similar rate as wild-type animals.

The delayed midgut histolysis phenotype seen in animals expressing Atg18^GD12342 under the control of Scer\GAL4^NP1 after puparium formation is not enhanced by co-expression of decay^GD8484.

The delayed midgut histolysis phenotype seen in animals expressing Atg1^GD7149 under the control of Scer\GAL4^NP1 after puparium formation is not enhanced by co-expression of decay^GD8484.

(Denton et al., 2009)

Xenogenetic Interactions

Statement

Reference

Pupae co-expressing decay^GD8484 and BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^NP1 undergo midgut histolysis at a similar rate as wild-type animals.

(Denton et al., 2009)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v43029

w¹¹¹⁸; P{GD8484}v43029

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8484

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Decay^GD8484

decay^GD8484

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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