UASt regulatory sequences drive expression of an inverted repeat.
Adults expressing Ubc6GD13294 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in flightless flies or late pupal lethality.
Expression under the control of Scer\GAL4Mef2.PR results in missing adult indirect flight muscles.
Expression under the control of Scer\GAL4Mef2.PR results in degenerate myofibrils in the adult indirect flight muscles.
Expression under the control of Scer\GAL4Mef2.PR results in sarcomeres with broadened Z-lines in the adult indirect flight muscles.
Co-expression of Usp47GD10642 and Ubc6GD13294 under the control of Scer\GAL4en-e16E leads to extensive larval lethality and the wing discs from larvae that can be recovered have a reduced size compared to controls.