FlyBase Allele Report: Dmel\Dronc[GD12376]

General Information

Symbol

Dmel\Dronc^GD12376

Species

D. melanogaster

Name

FlyBase ID

FBal0207720

Feature type

allele

Associated gene

Dmel\Dronc

Associated Insertion(s)

Carried in Construct

P{GD12376}

Also Known As

UAS-Dronc-RNAi, UAS-Dronc RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12376}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12376

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Rab5^GD10492

(Ballesteros-Arias et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^ppk.1.9

(Tao and Rolls, 2011)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible | adult stage, with Scer\GAL4^ap-md544

(Shinoda et al., 2019)

Phenotype Manifest In

dendrite, with Scer\GAL4^ppk.1.9

(Tao and Rolls, 2011)

larval dorsal multidendritic neuron ddaC, with Scer\GAL4^ppk.1.9

(Tao and Rolls, 2011)

wing, with Scer\GAL4^ap-md544

(Shinoda et al., 2019)

Detailed Description

Statement

Reference

The expression of Dronc^GD12376 under the control of Scer\GAL4^ap-md544 leads to opaque but straight wings, which respectively indicate apoptosis but no growth inhibition during development. Upon additional Dicer-2 expression (for a more efficient RNAi), some wings become wrinkled or show blisters.

Expression under the control of Scer\GAL4^C10 does not affect the overall wing size.

(Shinoda et al., 2019)

Expression of Dronc^GD12376 under the control of Scer\GAL4^GMR.PF does not affect eye development.

(Nandi et al., 2014)

Pruning of ddaC dendrites is delayed upon expression of Nc^GD12376 under the control of Scer\GAL4^ppk.1.9. The degeneration of dendrites after severing is unaffected however.

(Tao and Rolls, 2011)

Adults expressing Dronc^GD12376 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12376

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Scer\GAL80^ts.αTub84B, Dronc^GD12376, Scer\GAL4^da.PU is a non-enhancer of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

Suppressor of

Statement

Reference

Dronc^GD12376, Scer\GAL4^en-e16E is a suppressor | partially of abnormal size | third instar larval stage phenotype of Scer\GAL4^en-e16E, dome^ΔCYT.UAS

(Recasens-Alvarez et al., 2017)

Dronc^GD12376, Scer\GAL4^en-e16E is a suppressor | partially of increased cell death | third instar larval stage phenotype of Scer\GAL4^en-e16E, dome^ΔCYT.UAS

(Recasens-Alvarez et al., 2017)

Dronc^GD12376, Scer\GAL4^salm.EPv is a suppressor of neoplasia | third instar larval stage phenotype of Rab5^GD10492, Scer\GAL4^salm.EPv

(Ballesteros-Arias et al., 2014)

Dronc^GD12376, Scer\GAL4^salm.EPv is a suppressor of increased cell death | third instar larval stage phenotype of Rab5^GD10492, Scer\GAL4^salm.EPv

(Ballesteros-Arias et al., 2014)

Scer\GAL4^salm.EPv/Dronc^GD12376 is a suppressor of increased occurrence of cell division | third instar larval stage phenotype of Rab5^GD10492

(Ballesteros-Arias et al., 2014)

NOT Suppressor of

Statement

Reference

Scer\GAL80^ts.αTub84B, Dronc^GD12376, Scer\GAL4^da.PU is a non-suppressor of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Dronc^GD12376, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Acn^UAS.cNa, Scer\GAL4^GMR.PF

(Nandi et al., 2014)

Suppressor of

Statement

Reference

Dronc^GD12376, Scer\GAL4^en-e16E is a suppressor | partially of wing disc posterior compartment | third instar larval stage phenotype of Scer\GAL4^en-e16E, dome^ΔCYT.UAS

(Recasens-Alvarez et al., 2017)

Dronc^GD12376, Scer\GAL4^salm.EPv is a suppressor of wing pouch | third instar larval stage phenotype of Rab5^GD10492, Scer\GAL4^salm.EPv

(Ballesteros-Arias et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of Dronc^GD12376 partially suppresses the severe decrease in size and the severe increase in apoptosis induced in the third instar larval wing disc posterior compartment by the expression of dome^{ΔCYT.Scer\UAS} under the control of Scer\GAL4^en-e16E.

(Recasens-Alvarez et al., 2017)

Co-expression of Dronc^GD12376 results in significant suppression of the increased apoptosis and increased proliferation of the mutant domain, preventing tumorigenesis in flies expressing Rab5^GD10492 under the control of Scer\GAL4^salm.EPv (in combination with a Gal80[ts] transgene to restrict expression to the third instar larval stage) in the wing pouch.

(Ballesteros-Arias et al., 2014)

Expression of Dronc^GD12376 in the developing eye does not affect the eye roughness seen upon expression of Acn^Scer\UAS.cNa under the control of Scer\GAL4^GMR.PF.

(Nandi et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v23033

w¹¹¹⁸; P{GD12376}v23033

v23035

w¹¹¹⁸; P{GD12376}v23035

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12376

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Dronc^GD12376

Nc^GD12376

dronc^KD

(Ballesteros-Arias et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (17)

Report Sections

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