FlyBase Allele Report: Dmel\Dcp-1[GD10729]

General Information

Symbol

Dmel\Dcp-1^GD10729

Species

D. melanogaster

Name

FlyBase ID

FBal0206823

Feature type

allele

Associated gene

Dmel\Dcp-1

Associated Insertion(s)

Carried in Construct

P{GD10729}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD10729}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD10729

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal starvation stress response, with Scer\GAL4^da.G32

(Nandi et al., 2014)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible | adult stage, with Scer\GAL4^ap-md544

(Shinoda et al., 2019)

Phenotype Manifest In

autophagosome, with Scer\GAL4^da.G32

(Nandi et al., 2014)

wing, with Scer\GAL4^ap-md544

(Shinoda et al., 2019)

Detailed Description

Statement

Reference

The expression of Dcp-1^GD10729 under the control of Scer\GAL4^ap-md544 alone does not lead to obvious wing defects. Upon additional Dicer-2 expression (for a more efficient RNAi), wings become curled and opaque, which is indicative of growth inhibition and apoptosis during development, respectively.

(Shinoda et al., 2019)

Expression of Dcp-1^GD10729 in the developing eye, under the control of Scer\GAL4^GMR.PF does not affect eye development.

Knockdown of Dcp-1 through expression of Dcp-1^GD10729 in the fat bodies, under the control of Scer\GAL4^da.G32 induces a higher level of autophagy in fed larvae, compared to wild-type. The level of autophagy is increased further upon starvation.

(Nandi et al., 2014)

Adults expressing Dcp-1^GD10729 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10729

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Dcp-1^GD10729, Scer\GAL80^ts.αTub84B, Scer\GAL4^da.PU is a non-enhancer of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

NOT Suppressor of

Statement

Reference

Dcp-1^GD10729, Scer\GAL80^ts.αTub84B, Scer\GAL4^da.PU is a non-suppressor of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Dcp-1^GD10729, Scer\GAL4^da.G32 has autophagosome phenotype, suppressible by Acn^KK111555, Scer\GAL4^da.G32

(Nandi et al., 2014)

Enhancer of

Statement

Reference

Dcp-1^GD10729, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Acn^UAS.cNa, Scer\GAL4^GMR.PF

(Nandi et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Dcp-1^GD10729 enhances the rough eye phenotype and reduces the overall eye size in flies expressing Acn^Scer\UAS.cNa under the control of Scer\GAL4^GMR.PF.

Fed or starved larvae experiencing knockdown of both Dcp-1 and Acn (through expression of Dcp-1^GD10729 and Acn^KK111555 under the control of Scer\GAL4^da.G32), display wild-type levels of autophagy, indicating that the remaining protein after Acn knockdown is sufficient to support starvation-induced autophagy but not sufficient to drive elevated levels of basal autophagy in a fed Dcp-1 background.

(Nandi et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v34328