FlyBase Allele Report: Dmel\fwe[GD4616]

General Information

Symbol

Dmel\fwe^GD4616

Species

D. melanogaster

Name

FlyBase ID

FBal0204968

Feature type

allele

Associated gene

Dmel\fwe

Associated Insertion(s)

Carried in Construct

P{GD4616}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4616}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4616

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell death | recessive | somatic clone, with Scer\GAL4^αTub84B.PBb

(Rhiner et al., 2010)

increased cell death | somatic clone, with Scer\GAL4^Act5C.PI

(Rhiner et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adults expressing fwe^GD4616 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

In competition with dm^αTub84B.PBb expressing cells, cell clones expressing fwe^GD4616 driven by Scer\GAL4^αTub84B.PBb survive significantly better than wild-type cells in a growing population of wing imaginal disc cells.

Clones of cells expressing fwe^GD4616 under the control of Scer\GAL4^Act5C.PI in the wing imaginal disc epithelium tend to die 96 hours after clone induction when surrounded by wild-type cells.

Cells of the posterior compartment of the wing imaginal disc expressing fwe^GD4616 under the control of Scer\GAL4^en-e16E do not show signs of increased apoptosis compared to controls. Compartment size is not affected by expression of fwe^GD4616 under the control of Scer\GAL4^en-e16E.

(Rhiner et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 10-20% of the Scer\GAL4^pnr-MD237 expression domain.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4616

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

fwe^GD4616/Scer\GAL4^GMR.PF is a non-enhancer of increased cell death phenotype of Scer\GAL4^GMR.PF, egr^UAS.cMa

(Rhiner et al., 2010)

Suppressor of

Statement

Reference

Scer\GAL4^en-e16E/fwe^GD4616 is a suppressor of abnormal cell growth | somatic clone phenotype of Myc^αTub84B.PBb

(Rhiner et al., 2010)

NOT Suppressor of

Statement

Reference

fwe^GD4616/Scer\GAL4^GMR.PF is a non-suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF, egr^UAS.cMa

(Rhiner et al., 2010)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^en-e16E/fwe^GD4616 is a suppressor of wing disc | somatic clone phenotype of Myc^αTub84B.PBb

(Rhiner et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of fwe^GD4616 with egr^Scer\UAS.cMa driven by Scer\GAL4^GMR.PF has no effect on the eye ablation phenotype resulting from egr^Scer\UAS.cMa-overexpression.

Expression of fwe^GD4616 driven by Scer\GAL4^en-e16E significantly reduces the expansion of dm^αTub84B.PBb-overexpressing clones in the posterior compartment of wing imaginal discs.

(Rhiner et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v39596

w¹¹¹⁸; P{GD4616}v39596

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4616

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG6151^GD4616

flower^GD4616

fwe^GD4616

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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