UASt regulatory sequences drive expression of an inverted repeat.
Adults expressing kriGD11686 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
kriGD11686, Scer\GAL4GMR.PF is a suppressor of melanotic necrosis phenotype of Hsap\HTT152Q.Ex1.UAS.EGFP, Hsap\HTT48Q.Ex1.UAS.EGFP.Tag:NLS(Unk), Scer\GAL4GMR.PF
kriGD11686, Scer\GAL4elav-C155 is a suppressor of abnormal neuroanatomy phenotype of Hsap\HTT98Q.Ex1.UAS.EGFP.Tag:NLS(Unk), Scer\GAL4elav-C155
kriGD11686, Scer\GAL4GMR.PF is a suppressor of eye phenotype of Hsap\HTT152Q.Ex1.UAS.EGFP, Hsap\HTT48Q.Ex1.UAS.EGFP.Tag:NLS(Unk), Scer\GAL4GMR.PF
kriGD11686, Scer\GAL4GMR.PF is a suppressor of pigment cell phenotype of Hsap\HTT152Q.Ex1.UAS.EGFP, Hsap\HTT48Q.Ex1.UAS.EGFP.Tag:NLS(Unk), Scer\GAL4GMR.PF
Expression of CG5537GD11686 in the developing eye under the control of Scer\GAL4GMR.PF suppresses the loss of pigment seen in mutant flies co-expressing Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP and Hsap\HTT48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 under the control of Scer\GAL4GMR.PF. There is a clear decrease in the number of necrotic fly eyes in these progeny.
Co-expression of CG5537GD11686 in a Hsap\HTT98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 mutant brain (with both transgenes under the control of Scer\GAL4elav-C155) significantly decreases the average number and size of brain aggregate inclusions compared to flies expressing Hsap\HTT98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 alone.