FlyBase Allele Report: Dmel\5-HT1B[UAS.cYa]

General Information

Symbol

Dmel\5-HT1B^UAS.cYa

Species

D. melanogaster

Name

FlyBase ID

FBal0194588

Feature type

allele

Associated gene

Dmel\5-HT1B

Associated Insertion(s)

Carried in Construct

P{UAS-5-HT1B.Y}

Also Known As

UAS-d5-HT1B

Key Links

Transgenic product class

wild_type

(Yuan et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Yuan et al., 2005)

Mutagen

in vitro construct

(Yuan et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-5-HT1B.Y}

Cytology

Description

UAS regulatory sequences drive expression of 5-HT1B coding sequences.

(Yuan et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

5-HT1B

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | adult stage

(Mohammad et al., 2016)

abnormal circadian rhythm, with Scer\GAL4^5-HT1B.PY

(Yuan et al., 2005)

abnormal circadian rhythm, with Scer\GAL4^elav-C155

(Yuan et al., 2005)

abnormal circadian rhythm, with Scer\GAL4^tim.PE

(Yuan et al., 2005)

abnormal phototaxis | adult stage, with Scer\GAL4^nSyb.PS

(Mohammad et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

Pan-neuronal expression of 5-HT1B^Scer\UAS.cYa in adult flies (using 31[o]C heat-induced Scer\GAL4^nSyb.PS in combination with Scer\GAL80^ts.αTub84B) significantly decreases wall following behavior in an arena and decreases the percentage of time spent in a shaded area in a light or dark choice assay (posited measures of decreased anxiety), compared to non-induced (18[o]C) controls; walking speed is significantly decreased in flies both with and without the Scer\GAL4^nSyb.PS driver.

(Mohammad et al., 2016)

Expression of 5-HT1B^Scer\UAS.cYa under the control of Scer\GAL4^5-HTR1B does not affect either the total amount of sleep or sleep consolidation, measured as the average length of sleep bouts.

(Yuan et al., 2006)

Expression of 5-HT1B^Scer\UAS.cYa in clock neurons, under the control of Scer\GAL4^tim.PE, significantly reduces the magnitude of the phase shift induced by a short light pulse in the late (CT20) as well as in the early subjective night (CT15). Expression of 5-HT1B^Scer\UAS.cYa under the control of Scer\GAL4^elav-C155 or Scer\GAL4^5-HT1B also reduces phase shift, although to a lesser extent. Flies carrying two copies of 5-HT1B^Scer\UAS.cYa and two copies of Scer\GAL4^5-HT1B exhibit reduced phase shifts, to a similar extent as those with single copies of 5-HT1B^Scer\UAS.cYa and Scer\GAL4^tim.PE. Expression of 5-HT1B^Scer\UAS.cYa under the control of Scer\GAL4^GMR.PF has no such effect, suggesting that central clock neurons, instead of photoreceptor cells in the eye, are the relevant target cells of this inhibitory effect.

The rest-activity rhythms of 5-HT1B^Scer\UAS.cYa expressing flies (under the control of Scer\GAL4^tim.PE) in light-dark and dark-dark cycles are similar to those of wild-type flies. Overexpression of 5-HT1B^Scer\UAS.cYa does not affect per cycling in free-running conditions.

Flies overexpressing 5-HT1B^Scer\UAS.cYa under the control of Scer\GAL4^tim.PE exhibit increased rhythmicity in constant bright light (2500 lux), compared to controls. The difference is more significant in weaker illumination (250 lux).

(Yuan et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

5-HT1B^RNAi.UAS, 5-HT1B^UAS.cYa, Scer\GAL4^tim.PE has abnormal circadian rhythm phenotype, suppressible by cry^b, Scer\GAL4^tim.PE

(Yuan et al., 2005)

Suppressor of

Statement

Reference

5-HT1B^UAS.cYa, Scer\GAL4^tim.PE is a suppressor | partially of abnormal circadian rhythm phenotype of Scer\GAL4^tim.PE, sgg^UAS.cBa

(Yuan et al., 2005)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{Mef2.247.Switch}/5-HT1B^UAS.cYa is a non-suppressor of abnormal behavior phenotype of 5-HT1A^Δ5kb

(Yuan et al., 2006)

Other

Statement

Reference

5-HT1B^UAS.cYa, Scer\GAL4^tim.PE, cry^b has abnormal circadian rhythm phenotype

(Yuan et al., 2005)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of 5-HT1B^Scer\UAS.cYa, under the control of Scer\GAL4^{Mef2.247.Switch} does not rescue the 5-HT1A^Δ5kb sleep phenotype.

(Yuan et al., 2006)

Overexpression of 5-HT1B^Scer\UAS.cYa under the control of Scer\GAL4^tim.PE in a cry^b homozygous background does not have a discernible effect on the flies light sensitivity due to the strong phenotype produced by the cry^b mutation. However, ~87% of the cry^b heterozygotes overexpressing 5-HT1B^Scer\UAS.cYa under the control of Scer\GAL4^tim.PE are rhythmic in constant bright light, while only 13% of the control flies are rhythmic under these conditions. Fast Fourier Transform analysis indicates that cry^b heterozygous flies overexpressing 5-HT1B^Scer\UAS.cYa under the control of Scer\GAL4^tim.PE have very strong behavioural rhythms in constant bright light, although the average period is lengthened to about 28 hours.

Reducing 5-HT1B expression, through induction of 5-HT1B^IR.Scer\UAS, under the control of Scer\GAL4^tim.PE, or increasing cry levels through cry^Scer\UAS.cDa expression, eliminates the strong behavioural rhythms in constant bright light observed in cry^b heterozygous flies overexpressing 5-HT1B^Scer\UAS.cYa under the control of Scer\GAL4^tim.PE.

A large number (~43%) of cry^b flies overexpressing 5-HT1B^Scer\UAS.cYa under the control of Scer\GAL4^tim.PE are arrhythmic in dark-dark conditions, as compared to 7% in control cry^b flies.

(Yuan et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

27632

w^*; P{UAS-5-HT1B.Y}2

27633

w^*; P{UAS-5-HT1B.Y}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

5-HT1B^Scer\UAS.cYa

5-HT1B^UAS.cYa

d5-HT1B

(Yuan et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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