FB2024_03 , released June 25, 2024
Allele: Dmel\Ppt1RNAi.UAS
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General Information
Symbol
Dmel\Ppt1RNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0193176
Feature type
allele
Associated gene
Dmel\Ppt1
Associated Insertion(s)
Carried in Construct
P{UAS-Ppt1.RNAi}
Key Links
Transgenic product class
RNAi_reagent
(Hickey et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Hickey et al., 2006)
Mutagen
in vitro construct
(Hickey et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-Ppt1.RNAi}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat of the first 800bp of the Ppt1 cDNA separated by a short spacer.

(Hickey et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Ppt1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  neuronal ceroid lipofuscinosis 1
      CEC
      (Hickey et al., 2006)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 1 )
       

      In humans, Batten disease is characterised by the accumulate abnormal storage material in irregular granular deposits (GRODs) and autofluorescent inclusions in the brain, and the progressive death of neurons in the CNS. The Ppt1dsRNA.Scer\UAS, Ppt1 knockdown flies show abnormal storage material in highly laminar spherical deposits and increased autofluorescent inclusions.

      (Hickey et al., 2006)
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing Ppt1dsRNA.Scer\UAS under the control of Scer\GAL4Act5C.PI accumulate abnormal laminar deposits in their brains.

      (Hickey et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Used to study the phenotypic consequences of dsRNA interference (RNAi) of the Ppt1 gene.

      (Hickey et al., 2006)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Ppt1RNAi.UAS
      Ppt1dsRNA.Scer\UAS
      Ppt1dsRNA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)