A nuclear export signal (Tag:NES(HIV-1-Rev)) has been added. The construct has 77 Q repeats.
When Hsap\MJDQ77.Scer\UAS.T:Ivir\HA1,T:HIV-1\nes is driven by Scer\GAL4Appl.G1a or Scer\GAL4179Y large numbers of organelle accumulations are seen within larval nerves. No neuronal apoptosis is seen, and the larvae die as second or third instar. When Hsap\MJDQ77.Scer\UAS.T:Ivir\HA1,T:HIV-1\nes is driven in the eye by Scer\GAL4GMR.PS a severe degenerative eye phenotype is seen.
Hsap\ATXN3Q77.UAS.Tag:HA,Tag:NES(HIV-1-Rev), Scer\GAL4179Y has lethal | larval stage phenotype, enhanceable by Df(2R)k10408
Hsap\ATXN3Q77.UAS.Tag:HA,Tag:NES(HIV-1-Rev), Scer\GAL4Appl.G1a has lethal | larval stage phenotype, enhanceable by Df(2R)k10408
Hsap\ATXN3Q77.UAS.Tag:HA,Tag:NES(HIV-1-Rev), Scer\GAL4179Y has axon phenotype, enhanceable by Khc9
Hsap\ATXN3Q77.UAS.Tag:HA,Tag:NES(HIV-1-Rev), Scer\GAL4Appl.G1a has axon phenotype, enhanceable by Khc9
Hsap\ATXN3Q77.UAS.Tag:HA,Tag:NES(HIV-1-Rev), Scer\GAL4179Y has axon phenotype, suppressible by Hsc70-4UAS.cEa, Scer\GAL4179Y
Hsap\ATXN3Q77.UAS.Tag:HA,Tag:NES(HIV-1-Rev), Scer\GAL4Appl.G1a has axon phenotype, suppressible by Hsc70-4UAS.cEa, Scer\GAL4Appl.G1a