Construct: An Hsp70 promoter is fused upstream of an incomplete P-element, "KP", which is a 1.15kb element known to encode a repressor polypeptide of 207 amino acids. The "KP" element has a deletion from nucleotide 803 to 2564 and nucleotide 2866A is also deleted (coordinates relative to the canonical P-element described in FBrf0038996). The intact P-element promoter is present in this fragment.
H{hsp/KP} represses the P\TCP.hs transposase activity encoded by H{hsp/CP}2; the rate of snw mutation in the male or female germline caused by H{hsp/CP}2 is reduced if H{hsp/KP} is present. H{hsp/KP} cannot effectively repress the somatic transposase activity of P{Δ2-3}99B. H{hsp/KP} represses the transposase activity of P{Δ2-3}99B in the germline; the rate of snw mutation in the male germline caused by P{Δ2-3}99B is reduced if H{hsp/KP} is present. The repression of transposase activity by H{hsp/KP} appears to be due to a zygotic effect. There is no evidence for repression by a strictly maternal effect.
