FlyBase Allele Report: Dmel\Gαi[Q205L.UAS]

General Information

Symbol

Dmel\Gαi^Q205L.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0128189

Feature type

allele

Associated gene

Dmel\Gαi

Associated Insertion(s)

Carried in Construct

P{UAS-Gαi.Q205L}

Also Known As

UAS-Gαi^Q205L, UAS-GαiQ205L

Key Links

Transgenic product class

missense_variant

(Schaefer et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Schaefer et al., 2001)

Mutagen

in vitro construct

(Fuse et al., 2003, Schaefer et al., 2001)

Progenitor genotype

Gαi^UAS.cFa

(Fuse et al., 2003)

Carried in construct

P{UAS-Gαi.Q205L}

Cytology

Description

UASt regulatory sequences drive expression of mutated Gαi (carries the amino acid replacement Q205L).

(Schaefer et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Gαi

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle, with Scer\GAL4^{VP16.mat.αTub67C}

(Yu et al., 2005)

abnormal neuroanatomy, with Scer\GAL4^{VP16.mat.αTub67C}

(Yu et al., 2005)

Phenotype Manifest In

neuroblast, with Scer\GAL4^{VP16.mat.αTub67C}

(Yu et al., 2005)

sensory mother cell & spindle, with Scer\GAL4^sca-109-68

(Schaefer et al., 2001)

Detailed Description

Statement

Reference

The response of the ab1C neuron to CO[[2]] is unaffected in animals expressing G-iα65A^{Q205L.Scer\UAS} under the control of Scer\GAL4^Gr21a.9.323.

The odour responses of the ab1A, ab2A and ab3A olfactory receptor neurons are unaffected in animals expressing G-iα65A^{Q205L.Scer\UAS} under the control of Scer\GAL4^{Orco.T:Hsim\VP16}.

(Yao and Carlson, 2010)

Expression of G-iα65A^{Q205L.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096 does not produce any bristle phenotype.

(Katanaev and Tomlinson, 2006)

Expression of G-iα65A^Scer\UAS.cLa in glia of embryos, driven by Scer\GAL4^repo, does not cause a phenotype.

(Schwabe et al., 2005)

Expression of G-iα65A^{Q205L.Scer\UAS}, under the regulation of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} generates symmetric division in 2% of telophase neuroblasts, as oppose to asymmetrically as in wild-type.

(Yu et al., 2005)

Embryos expressing G-iα65A^{Q205L.Scer\UAS} under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} show no defects in spindle orientation in neuroblasts. Expression of G-iα65A^{Q205L.Scer\UAS} under the control of Scer\GAL4^sca-109-68 results in defects in spindle orientation in the sensory organ precursor cells.

(Schaefer et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

G-iα65A^{Q205L.Scer\UAS}

Gαi^{Q205L.Scer\UAS}

Gαi^Q205L.UAS

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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