FB2024_03 , released June 25, 2024
Allele: Dmel\dppUAS.E.EGFP
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General Information
Symbol
Dmel\dppUAS.E.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0120516
Feature type
allele
Associated gene
Dmel\dpp
Associated Insertion(s)
Carried in Construct
P{UAS-dpp.GFP.E}
Also Known As
UAS-GFP-Dpp, UAS-Dpp-GFP, GFP-dpp
Key Links
Transgenic product class
cDNA
(Entchev et al., 2000)
wild_type
(Entchev et al., 2000)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Entchev et al., 2000)
wild_type
(Entchev et al., 2000)
Mutagen
in vitro construct
(Entchev et al., 2000)
Progenitor genotype
Carried in construct
P{UAS-dpp.GFP.E}
Cytology
Description

UAS regulatory sequences drive expression of a dpp cDNA which is tagged at codon 465 with EGFP.

(Entchev et al., 2000)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
dpp
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of dppUAS.E.EGFP under the control of Scer\GAL4Mhc.PW leads to a decrease in the spontaneous locomotion and in the probability of proboscis extension reflex (PER) when compared to control adults. Its expression under the control of Scer\GAL4GS.Act88F in RU486 fed animals leads to a decrease in the spontaneous locomotion particularly in proximity to the food and an increase in the probability of PER when compared to control adults.

      (Robles-Murguia et al., 2020)

      Overexpression of dppScer\UAS.E.T:Avic\GFP-EGFP in the wing disc, under the control of Scer\GAL4dpp.blk1 results in pupal lethality.

      (Michel et al., 2011)

      Eggshells generated from mothers that overexpress dppScer\UAS.E.T:Avic\GFP-EGFP under the control of Scer\GAL455B at 290C in anterior follicle cells show an expanded operculum.

      (Carneiro et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      A Sec152 heterozygous background partially suppresses the pupal lethality caused by Scer\GAL4dpp.blk1-mediated overexpression of dppScer\UAS.E.T:Avic\GFP-EGFP.

      (Michel et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      dppUAS.E.EGFP/Scer\GAL4dpp.blk1 partially rescues dppd14/dppd12

      (Entchev et al., 2000)
      Comments

      dppScer\UAS.E.T:Avic\GFP-EGFP expressed under the control of Scer\GAL4dpp.blk1 restores normal epidermal structures in dppd12/dppd14 flies. These flies fail to hatch from the pupal case.

      (Entchev et al., 2000)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      dppScer\UAS.E.T:Avic\GFP-EGFP
      dppScer\UAS.E.T:Avic\GFP-F64L.S65T
      dppUAS.E.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (21)