Construct: Expression of the Pc coding region, contained within a 1.6kb EcoRI-DraI fragment, is governed by a heat shock promoter.
Pchs.PF is a suppressor | heat sensitive | partially of chemical resistant phenotype of Scer\GAL4hry.PU, Tn\neoRUAS.GFP
The ability of animals expressing Tn\neoRScer\UAS.T:Avic\GFP under the control of Scer\GAL4h.PU to survive in the presence of a concentration of G418 which is lethal to wild-type controls is partially suppressed if they also express Pchs.PF (using heat shock).