FB2024_04 , released June 25, 2024
Allele: Dmel\E(spl)m4-BFMUAS.cAa
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General Information
Symbol
Dmel\E(spl)m4-BFMUAS.cAa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Apidianakis
FlyBase ID
FBal0101268
Feature type
allele
Associated gene
Dmel\E(spl)m4-BFM
Associated Insertion(s)
Carried in Construct
P{UAS-E(spl)m4-BFM.A}
Also Known As
UAS-m4
Key Links
Transgenic product class
cDNA
(Apidianakis et al., 1999)
wild_type
(Apidianakis et al., 1999)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Apidianakis et al., 1999)
wild_type
(Apidianakis et al., 1999)
Mutagen
in vitro construct
(Apidianakis et al., 1999)
Progenitor genotype
Carried in construct
P{UAS-E(spl)m4-BFM.A}
Cytology
Description

The complete E(spl)m4-BFM cDNA is under the control of UASt sequences.

(Apidianakis et al., 1999)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
E(spl)m4-BFM
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      campaniform sensillum, with Scer\GAL4455.2

      (Apidianakis et al., 1999)

      campaniform sensillum, with Scer\GAL4ap-md544

      (Apidianakis et al., 1999)

      campaniform sensillum, with Scer\GAL4pnr-MD237

      (Apidianakis et al., 1999)

      campaniform sensillum, with Scer\GAL4ptc-559.1

      (Apidianakis et al., 1999)

      campaniform sensillum, with Scer\GAL4sca-T3

      (Apidianakis et al., 1999)

      campaniform sensillum of anterior crossvein, with Scer\GAL4ptc-559.1

      (Apidianakis et al., 1999)

      campaniform sensillum of anterior crossvein | precursor, with Scer\GAL4ap-md544

      (Apidianakis et al., 1999)

      campaniform sensillum of dorsal radius, with Scer\GAL4ap-md544

      (Apidianakis et al., 1999)

      dorsal row & sensory mother cell, with Scer\GAL4ap-md544

      (Apidianakis et al., 1999)

      macrochaeta, with Scer\GAL4455.2

      (Apidianakis et al., 1999)

      macrochaeta, with Scer\GAL4ap-md544

      (Apidianakis et al., 1999)

      macrochaeta, with Scer\GAL4pnr-MD237

      (Apidianakis et al., 1999)

      macrochaeta, with Scer\GAL4ptc-559.1

      (Apidianakis et al., 1999)

      macrochaeta, with Scer\GAL4sca-T3

      (Apidianakis et al., 1999)

      microchaeta, with Scer\GAL4455.2

      (Apidianakis et al., 1999)

      microchaeta, with Scer\GAL4ap-md544

      (Apidianakis et al., 1999)

      microchaeta, with Scer\GAL4pnr-MD237

      (Apidianakis et al., 1999)

      microchaeta, with Scer\GAL4ptc-559.1

      (Apidianakis et al., 1999)

      microchaeta, with Scer\GAL4sca-T3

      (Apidianakis et al., 1999)

      microchaeta | somatic clone, with Scer\GAL4Ubx.PdC

      (Apidianakis et al., 1999)

      scutellar bristle, with Scer\GAL4455.2

      (Apidianakis et al., 1999)

      scutellar bristle, with Scer\GAL4ptc-559.1

      (Apidianakis et al., 1999)

      scutellar bristle | ectopic, with Scer\GAL4455.2

      (Apidianakis et al., 1999)

      sensory mother cell | ectopic, with Scer\GAL4bi-omb-Gal4

      (Nagel et al., 2000)
      Detailed Description
      Statement
      Reference

      No extra neuroblasts are seen when E(spl)m4-BFMScer\UAS.cAa is expressed under the control of Scer\GAL4wor.PA or Scer\GAL4elav.PU.

      (San-Juán and Baonza, 2011)

      Expression of m4Scer\UAS.cAa under the control of Scer\GAL4ey.PB has no effect on either eye or antenna disc development.

      (Kumar and Moses, 2001)

      Expression of m4Scer\UAS.cAa when driven by Scer\GAL4bi-omb-Gal4 in the wing disc leads to an increase in the numbers of sensory organ precursors seen.

      (Nagel et al., 2000)

      Flies with m4Scer\UAS.cAa under the control of Scer\GAL4ptc-559.1, Scer\GAL4sca-T3, Scer\GAL4455.2, Scer\GAL4pnr-MD237 or Scer\GAL4ap-md544, show duplications of sensillum campaniformium, macrochaetae and microchaetae in their normal locations that are frequently tufted together. No effects are seen in the wing. Flies with m4Scer\UAS.cAa under the control of Scer\GAL4ptc-559.1, show duplications of the scutellar bristles and the anterior cross-vein campaniform sensilla in their normal locations. If two copies of m4Scer\UAS.cAa are present the number of bristles increases yet more. Flies with m4Scer\UAS.cAa under the control of Scer\GAL4455.2, show duplications of the scutellar bristles. Flies with m4Scer\UAS.cAa under the control of Scer\GAL432B or Scer\GAL4bi-md653 showed no wing pattern aberrations. m4Scer\UAS.cAa, when driven by Scer\GAL4ap-md544, creates clusters of wing margin sensory organ precursors (SOPs) in their normal locations in the dorsal row, where there would only be single SOPs in wild-type. Over commitment of the ACV and L3 precursors of campaniform sensilla are also seen. The N/numb mediated asymmetric divisions in the SOP lineage are not affected. In somatic clones where m4Scer\UAS.cAa is over-expressed under the control of Scer\GAL4Ubx.PdC, clustered microchaetae are seen, even at the clone boundary, suggesting the effect is acting at the level of signal reception.

      (Apidianakis et al., 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      mαScer\UAS.cAa enhances the number of extra scutellar bristles caused by expression of m4Scer\UAS.cAa under the control of Scer\GAL4ptc-559.1 m4Scer\UAS.cAa driven by Scer\GAL4ptc-559.1 cannot restore scutellar bristles to E(spl)Scer\UAS.cLa, HLHm7Scer\UAS.cdCa, HLHmδScer\UAS.cdCa or HLHmβScer\UAS.cdCa The scutellar bristle duplication phenotype caused by expression of m4Scer\UAS.cAa under the control of Scer\GAL4455.2 can be suppressed by a single copy of HLHm7Scer\UAS.cdCa. m4Scer\UAS.cAa, when driven by Scer\GAL4ap-md544 in a Df(1)sc10-1 background, does not restore any chaetae lost in this deficiency. The presence of NAx-1 partially suppresses the supernumerary scutellar bristle phenotype seen in m4Scer\UAS.cAa when driven by Scer\GAL4455.2. The presence of H2 partially suppresses the supernumerary scutellar bristle phenotype seen in m4Scer\UAS.cAa when driven by Scer\GAL4ptc-559.1. The presence of Df(3R)Dl-BX6, neurA101, bib2 or mami511 has no significant effect on the supernumerary scutellar bristle phenotype seen in m4Scer\UAS.cAa when driven by Scer\GAL4ptc-559.1. The presence of Df(3R)grob32.2 enhances the supernumerary scutellar bristle phenotype seen in m4Scer\UAS.cAa when driven by Scer\GAL4ptc-559.1.

      (Apidianakis et al., 1999)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      E(spl)m4-BFMScer\UAS.cAa
      E(spl)m4-BFMUAS.cAa
      m4Scer\UAS.cAa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Apidianakis
      (Apidianakis et al., 1999)
      Secondary FlyBase IDs
        References (9)