FlyBase Allele Report: Avic\GFP[UAS.S65T.I167T.Tag:MT(btau)]

General Information

Symbol

Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}

Species

A. victoria

Name

FlyBase ID

FBal0098532

Feature type

allele

Associated gene

Avic\GFP

Associated Insertion(s)

Carried in Construct

P{UAS-Tau-GFP.S65T.I167T}

Also Known As

UAS-Tau-GFP, tau-GFP

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Murray et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-Tau-GFP.S65T.I167T}

Cytology

Description

UASt regulatory sequences drive expression of the GFP(S65T.I167T) fluorescent protein tagged with Tag:MT(btau).

(Murray et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

GFP(S65T.I167T)

green fluorescent protein

Tagged with

Tag:MT(btau)

microtubule protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^smid-C161

(Williams et al., 2000)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^109(2)80

(Lin et al., 2010)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^ppk.PG

(Lin et al., 2010)

Phenotype Manifest In

dendrite | third instar larval stage, with Scer\GAL4^109(2)80

(Lin et al., 2010)

dendrite | third instar larval stage, with Scer\GAL4^ppk.PG

(Lin et al., 2010)

dendritic arborizing neuron | third instar larval stage, with Scer\GAL4^109(2)80

(Lin et al., 2010)

larval multidendritic class IV neuron | third instar larval stage, with Scer\GAL4^ppk.PG

(Lin et al., 2010)

metathoracic neuromere, with Scer\GAL4^smid-C161

(Williams et al., 2000)

microtubule, with Scer\GAL4^ppk.PG

(Lin et al., 2010)

prothoracic femoral chordotonal organ & axon, with Scer\GAL4^smid-C161

(Williams et al., 2000)

prothoracic neuromere, with Scer\GAL4^en-e16E

(Williams et al., 2000)

prothoracic neuromere, with Scer\GAL4^smid-C161

(Williams et al., 2000)

Detailed Description

Statement

Reference

Expression of Avic\GFP^{Scer\UAS.S65T.I167T.T:Btau\MAPT} under the control of Scer\GAL4^109(2)80 in dendritic arborization (DA) neurons causes severe dendrite degeneration.

Expression of Scer\GAL4^ppk.PG>Avic\GFP^{Scer\UAS.S65T.I167T.T:Btau\MAPT} engenders microtubule structure defects in dendrites.

Expression of Avic\GFP^{Scer\UAS.S65T.I167T.T:Btau\MAPT} in class IV dendritic arborizing (DA) neurons under the control of Scer\GAL4^ppk.PG induces spheroid inclusion-like structures. These inclusions form preferentially at distal dendrites, although some are also present at proximal segments, causing local swelling.

(Lin et al., 2010)

Expression of Avic\GFP^{Scer\UAS.S65T.I167T.T:Btau\MAPT} under the control of Scer\GAL4^smid-C161 results in defects in the central axon projections in the prothoracic neuromere in adults. Asymmetry in the projections is seen. There are four broad categories of defects in the prothoracic neuromere; reduction or loss of whole axon projections, coalescence of axons to form abnormal bundles, under-elaboration of terminal arborisations and the formation of abnormal swellings and varicosities. The medial projection of the femoral chordotonal organ (FCO) is defective in most cases, either being completely absent or massively reduced. The cell bodies of the FCO neurons are intact and appear indistinguishable from those in controls. Abnormal bundling of the sensory axons is sometimes seen in the anterior projection in the prothoracic neuromere and in the posterior projection in the metathoracic neuromere. The medial projection of the FCO is completely normal in pupae 72 hours after puparium formation in animals expressing Avic\GFP^{Scer\UAS.S65T.I167T.T:Btau\MAPT} under the control of Scer\GAL4^smid-C161. Expression of Avic\GFP^{Scer\UAS.S65T.I167T.T:Btau\MAPT} under the control of Scer\GAL4^en-e16E results in defects in the projections in the prothoracic neuromere in adults. Defects include loss of axons, abnormal axon bundling and beading. Expression of Avic\GFP^{Scer\UAS.S65T.I167T.T:Btau\MAPT} under the control of Scer\GAL4^C42 results in axon defects identical to those caused by expression of Avic\GFP^{Scer\UAS.S65T.I167T.T:Btau\MAPT} under the control of Scer\GAL4^smid-C161.

(Williams et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}, Scer\GAL4^109(2)80 has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by Hsap\LRRK2^{G2019S.UAS.cLa.Tag:FLAG}, Scer\GAL4^109(2)80

(Lin et al., 2010)

Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}, Scer\GAL4^ppk.PG has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by Hsap\LRRK2^{G2019S.UAS.cLa.Tag:FLAG}, Scer\GAL4^ppk.PG

(Lin et al., 2010)

Suppressed by

Statement

Reference

Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}, Scer\GAL4^109(2)80 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by Lrrk[+]/Lrrk^e03680

(Lin et al., 2010)

Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}, Scer\GAL4^ppk.PG has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by Lrrk[+]/Lrrk^e03680

(Lin et al., 2010)

Enhancer of

Statement

Reference

Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}, Scer\GAL4^109(2)80 is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\LRRK2^{G2019S.UAS.cLa.Tag:FLAG}, Scer\GAL4^109(2)80

(Lin et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}, Scer\GAL4^109(2)80 has dendritic arborizing neuron | third instar larval stage phenotype, enhanceable by Hsap\LRRK2^{G2019S.UAS.cLa.Tag:FLAG}, Scer\GAL4^109(2)80

(Lin et al., 2010)

Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}, Scer\GAL4^109(2)80 has dendrite | third instar larval stage phenotype, enhanceable by Hsap\LRRK2^{G2019S.UAS.cLa.Tag:FLAG}, Scer\GAL4^109(2)80

(Lin et al., 2010)

Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}, Scer\GAL4^ppk.PG has microtubule phenotype, enhanceable by Hsap\LRRK2^{G2019S.UAS.cLa.Tag:FLAG}, Scer\GAL4^ppk.PG

(Lin et al., 2010)

Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}, Scer\GAL4^ppk.PG has dendrite | third instar larval stage phenotype, enhanceable by Hsap\LRRK2^{G2019S.UAS.cLa.Tag:FLAG}, Scer\GAL4^ppk.PG

(Lin et al., 2010)

Suppressed by

Statement

Reference

Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}, Scer\GAL4^109(2)80 has dendritic arborizing neuron | third instar larval stage phenotype, suppressible | partially by Lrrk[+]/Lrrk^e03680

(Lin et al., 2010)

Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}, Scer\GAL4^109(2)80 has dendrite | third instar larval stage phenotype, suppressible | partially by Lrrk[+]/Lrrk^e03680

(Lin et al., 2010)

Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}, Scer\GAL4^ppk.PG has microtubule phenotype, suppressible | partially by Lrrk[+]/Lrrk^e03680

(Lin et al., 2010)

Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}, Scer\GAL4^ppk.PG has larval multidendritic class IV neuron | third instar larval stage phenotype, suppressible | partially by Lrrk[+]/Lrrk^e03680

(Lin et al., 2010)

Enhancer of

Statement

Reference

Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}, Scer\GAL4^109(2)80 is an enhancer of dendritic arborizing neuron | third instar larval stage phenotype of Hsap\LRRK2^{G2019S.UAS.cLa.Tag:FLAG}, Scer\GAL4^109(2)80

(Lin et al., 2010)

Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}, Scer\GAL4^109(2)80 is an enhancer of dendrite | third instar larval stage phenotype of Hsap\LRRK2^{G2019S.UAS.cLa.Tag:FLAG}, Scer\GAL4^109(2)80

(Lin et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\LRRK2^{G2019S.Scer\UAS.cLa.T:Zzzz\FLAG} strongly enhances the dendrite defects caused by Scer\GAL4^109(2)80>Avic\GFP^{Scer\UAS.S65T.I167T.T:Btau\MAPT}. In severe cases, only six of the eight dendritic arborization (DA) neuron somas are present in the dorsal field, an indication of neuronal loss that is not detected when Hsap\LRRK2^{G2019S.Scer\UAS.cLa.T:Zzzz\FLAG} or Avic\GFP^{Scer\UAS.S65T.I167T.T:Btau\MAPT} is expressed alone.

Co-expression of Avic\GFP^{Scer\UAS.S65T.I167T.T:Btau\MAPT} and Hsap\LRRK2^{G2019S.Scer\UAS.cLa.T:Zzzz\FLAG} under the control of Scer\GAL4^ppk.PG causes severe microtubule destruction in dendrites.

Co-expression of Hsap\LRRK2^{G2019S.Scer\UAS.cLa.T:Zzzz\FLAG} enhances the formation of spheroid inclusions caused by Scer\GAL4^ppk.PG>Avic\GFP^{Scer\UAS.S65T.I167T.T:Btau\MAPT}-expression. The spheroid inclusions appear much more frequent in both distal and proximal dendrites when Hsap\LRRK2^{G2019S.Scer\UAS.cLa.T:Zzzz\FLAG} is also expressed.

Lrrk^e03680/+ partially suppresses the dendrite degeneration phenotype resulting from the expression of Scer\GAL4^109(2)80>Avic\GFP^{Scer\UAS.S65T.I167T.T:Btau\MAPT}. Dendrites of the Avic\GFP^{Scer\UAS.S65T.I167T.T:Btau\MAPT}-expressing neurons are longer and the number of dendritic ends is increased. The suppression by Lrrk^e03680/+ is also observed in inclusion formation and microtubule fragmentation.

(Lin et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Avic\GFP^{Scer\UAS.S65T.I167T.T:Btau\MAPT}

Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}

Btau\MAPT^{Scer\UAS.T:Avic\GFP-S65T.I167T}

Name Synonyms

Secondary FlyBase IDs

References (9)

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