FlyBase Allele Report: Dmel\Pka-C1[UAS.cKa]

General Information

Symbol

Dmel\Pka-C1^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Kiger

FlyBase ID

FBal0098328

Feature type

allele

Associated gene

Dmel\Pka-C1

Associated Insertion(s)

Carried in Construct

P{UAS-Pka-C1.K}

Also Known As

UAS-PKAc

Key Links

Transgenic product class

wild_type

(Kiger et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Kiger et al., 1999)

Mutagen

in vitro construct

(Kiger et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-Pka-C1.K}

Cytology

Description

UASt regulatory sequences drive expression of wild type Pka-C1.

(Kiger et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Pka-C1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates obesity

modeled by Lsd-1¹

(Beller et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with Scer\GAL4^30A

(Kiger and Ho, 2001)

abnormal neuroanatomy, with Scer\GAL4^ppk.PG

(Copf, 2014)

lethal, with Scer\GAL4^KO5

(Kiger et al., 1999)

lethal, with Scer\GAL4^PP3

(Kiger et al., 1999)

partially lethal, with Scer\GAL4^e22c

(Kiger et al., 1999)

partially lethal, with Scer\GAL4^RK5

(Kiger et al., 1999)

visible, with Scer\GAL4^30A

(Kiger and Ho, 2001)

visible, with Scer\GAL4^RK5

(Kiger et al., 1999)

Phenotype Manifest In

adult, with Scer\GAL4^RK5

(Kiger et al., 1999)

dendrite, with Scer\GAL4^ppk.PG

(Copf, 2014)

dendritic branch point, with Scer\GAL4^ppk.PG

(Copf, 2014)

eye, with Scer\GAL4^GMR.PF

(Cho et al., 2001)

eye, with Scer\GAL4^RK5

(Kiger et al., 1999)

larval multidendritic class IV neuron, with Scer\GAL4^ppk.PG

(Copf, 2014)

macrochaeta, with Scer\GAL4^RK5

(Kiger et al., 1999)

melanotic mass, with Scer\GAL4^30A

(Kiger and Ho, 2001, Kiger et al., 2001)

nurse cell, with Scer\GAL4^nanos.PG

(Jackson and Berg, 2002)

wing, with Scer\GAL4^30A

(Kiger and Ho, 2001, Kiger et al., 2001)

wing, with Scer\GAL4^71B

(Kiger et al., 2001)

wing, with Scer\GAL4⁶⁸⁴

(Kiger et al., 2001)

wing, with Scer\GAL4^KO5

(Kiger et al., 1999)

wing, with Scer\GAL4^RK5

(Kiger et al., 1999)

wing vein | ectopic, with Scer\GAL4^71B

(Kiger et al., 2001)

wing vein | ectopic, with Scer\GAL4⁶⁸⁴

(Kiger et al., 2001)

Detailed Description

Statement

Reference

The class IV dendritic arborization neurons in flies expressing Pka-C1^Scer\UAS.cKa under the control of Scer\GAL4^ppk.PG show a significant decrease in the complexity of dendritic arbors in respect to the total dendritic length. The total number of dendritic branching points is also decreased.

(Copf, 2014)

Flies expressing Pka-C1^Scer\UAS.cKa under the control of Scer\GAL4^fat have a reduced fat content compared to controls.

(Beller et al., 2010)

1.2% of egg chambers have at least one binucleate nurse cell in females expressing Pka-C1^Scer\UAS.cKa under the control of Scer\GAL4^nos.PG.

(Jackson and Berg, 2002)

When Pka-C1^Scer\UAS.cKa is driven by Scer\GAL4^GMR.PF, the eye is discoloured and swelled up with wrinkles. The boundaries of all ommatidia and photoreceptor cells disappear.

(Cho et al., 2001)

Expression of Pka-C1^Scer\UAS.cKa under the control of Scer\GAL4^30A results in a collapsed wing phenotype. The flies have melanotic growths in the head and show a developmental delay of about 2 days compared to controls.

(Kiger and Ho, 2001)

When Pka-C1^Scer\UAS.cKa is driven by Scer\GAL4^30A, wings are variably unfolded or unfolded and blistered, or collapsed. All patterns elements of the wing (veins bristles and hairs) are normal. Also melanotic tumours are often seen within the head capsule. Melanotic tumours are also found in the wing or other parts of the body. Occasionally dead or moribund flies are found that have become entirely melanized after partially opening their wings. When Pka-C1^Scer\UAS.cKa is driven by Scer\GAL4⁶⁸⁴ or Scer\GAL4^71B, severe blistering of then wing blade is seen, accompanied by enlarged veins and a disrupted venation pattern.

(Kiger et al., 2001)

Expression of insertion P{UAS-Pka-C1.K}15.3 in backgrounds Scer\GAL4^RK5 and Scer\GAL4^KO5 allows partial survival; 35 with a sibling class of 47, and 24 with a sibling class of 129 respectively. In the Scer\GAL4^PP3 background 15.3 shows complete lethality. Expression of insertion P{UAS-Pka-C1.K}13.1 in the Scer\GAL4^RK5 background allows partial survival; 3 with a sibling class of 46. In the Scer\GAL4^PP3 and Scer\GAL4^KO5 backgrounds 13.1 shows complete lethality. Animals weakly expressing Pka-C1^Scer\UAS.cKa under the control of Scer\GAL4^RK5 sometimes die as blackened pharate adults, but adults that eclose and have a variety of overlapping phenotypes are also produced. These phenotypes include warped or collapsed wings, flecks on the surface of the eye and bristles glued to the thorax. Flies that are as small as half the normal size are also seen. Some show a combination of these defects, others were normal. In the Scer\GAL4^PP3 and Scer\GAL4^KO5 backgrounds P{UAS-Pka-C1.K}16.5 show complete lethality. Animals weakly expressing Pka-C1^Scer\UAS.cKa under the control of Scer\GAL4^KO5 sometimes give rise to adults that eclose but have collapsed wings. Expression of insertions of P{UAS-Pka-C1.K} show complete lethality in the Scer\GAL4^KO5, Scer\GAL4^PP3, and Scer\GAL4^RK5 backgrounds. Expression of insertions of P{UAS-Pka-C1.K} in a Scer\GAL4^e22c background that show the strongest phenotype usually fail to hatch, and the majority develop normal larval denticle belts.

(Kiger et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^Oamb.4.4/Pka-C1^UAS.cKa is a suppressor of female sterile | recessive phenotype of Octβ2R^f05679

(Lim et al., 2014)

Scer\GAL4^Oamb.4.4/Pka-C1^UAS.cKa is a suppressor | partially of decreased fecundity | recessive | female phenotype of Octβ2R^f05679

(Lim et al., 2014)

Other

Statement

Reference

Pka-C1^UAS.cKa, Scer\GAL4^30A, disco-r^bae-1 has lethal phenotype

(Kiger and Ho, 2001, Kiger et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A, pan^UAS.cWa has partially lethal phenotype

(Kiger et al., 2001)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Pka-C1^UAS.cKa, Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by Pdk1^EP837, Scer\GAL4^GMR.PF

(Cho et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-enhanceable by CrebB^Cbz.UAS, Scer\GAL4^30A

(Kiger et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-enhanceable by nej^Q7, Scer\GAL4^30A

(Kiger et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-enhanceable by pan², Scer\GAL4^30A

(Kiger et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-enhanceable by pan³, Scer\GAL4^30A

(Kiger et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-enhanceable by CrebB^S162, Scer\GAL4^30A

(Kiger et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-enhanceable by Stat92E⁰⁶³⁴⁶, Scer\GAL4^30A

(Kiger et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-enhanceable by Stat92E^j6C8, Scer\GAL4^30A

(Kiger et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-enhanceable by arm⁴, Scer\GAL4^30A

(Kiger et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-enhanceable by gro¹, Scer\GAL4^30A

(Kiger et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-enhanceable by gro^C105, Scer\GAL4^30A

(Kiger et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-enhanceable by nej³, Scer\GAL4^30A

(Kiger et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-enhanceable by nej^P, Scer\GAL4^30A

(Kiger et al., 2001)

Suppressed by

Statement

Reference

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, suppressible by pan^UAS.cWa, Scer\GAL4^30A

(Kiger et al., 2001)

NOT suppressed by

Statement

Reference

Pka-C1^UAS.cKa, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by Pdk1^EP837, Scer\GAL4^GMR.PF

(Cho et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-suppressible by CrebB^Cbz.UAS, Scer\GAL4^30A

(Kiger et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-suppressible by nej^Q7, Scer\GAL4^30A

(Kiger et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-suppressible by pan², Scer\GAL4^30A

(Kiger et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-suppressible by pan³, Scer\GAL4^30A

(Kiger et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-suppressible by CrebB^S162, Scer\GAL4^30A

(Kiger et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-suppressible by Stat92E⁰⁶³⁴⁶, Scer\GAL4^30A

(Kiger et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-suppressible by Stat92E^j6C8, Scer\GAL4^30A

(Kiger et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-suppressible by arm⁴, Scer\GAL4^30A

(Kiger et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-suppressible by gro¹, Scer\GAL4^30A

(Kiger et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-suppressible by gro^C105, Scer\GAL4^30A

(Kiger et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-suppressible by nej³, Scer\GAL4^30A

(Kiger et al., 2001)

Pka-C1^UAS.cKa, Scer\GAL4^30A has wing phenotype, non-suppressible by nej^P, Scer\GAL4^30A

(Kiger et al., 2001)

Suppressor of

Statement

Reference

Scer\GAL4^Oamb.4.4/Pka-C1^UAS.cKa is a suppressor | partially of egg | decreased number phenotype of Octβ2R^f05679

(Lim et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Pka-C1^Scer\UAS.cKa under the control of Scer\GAL4^fat suppresses the increased fat content seen in Lsd-1¹ flies.

(Beller et al., 2010)

The collapsed wing phenotype caused by expression of Pka-C1^Scer\UAS.cKa under the control of Scer\GAL4^30A is suppressed by the presence of the transcriptionally active P-element in the P{}cact^BQ insertion. The collapsed wing phenotype caused by expression of Pka-C1^Scer\UAS.cKa under the control of Scer\GAL4^30A is suppressed by the presence of the P-Repressor encoded by P{P-Sal}89D. Mosaic analysis using the P-Repressor encoded by P{P-Sal}89D to repress expression of Pka-C1^Scer\UAS.cKa under the control of Scer\GAL4^30A indicates that Pka-C1^Scer\UAS.cKa must be expressed for the lethal interaction between bae¹ and Pka-C1^Scer\UAS.cKa expressed under the control of Scer\GAL4^30A to take place.

(Kiger and Ho, 2001)

The addition of CrebB-17A^Cbz.Scer\UAS or CrebB-17A^S162 has little effect on the Pka-C1^Scer\UAS.cKa, Scer\GAL4^30A phenotypes. The addition of Stat92E⁰⁶³⁴⁶ or Stat92E^j6C8 has little effect on the Pka-C1^Scer\UAS.cKa, Scer\GAL4^30A phenotypes. The addition of Scer\GAL4^30A, strongly suppresses the wing phenotype caused by Pka-C1^Scer\UAS.cKa, Scer\GAL4^30A alone. Fewer survivors are seen than expected, though - and those flies are weak and are trapped easily in medium. The addition of bae¹ to Pka-C1^Scer\UAS.cKa, Scer\GAL4^30A flies is lethal.

(Kiger et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^Mef2.247/Pka-C1^UAS.cKa partially rescues Pka-C1^X4/Pka-C1^MB581

(Hong et al., 2008)

Scer\GAL4^c309/Pka-C1^UAS.cKa partially rescues Pka-C1^X4/Pka-C1^MB581

(Hong et al., 2008)

Comments

Expression of Pka-C1^Scer\UAS.cKa under the control of either Scer\GAL4^Mef2.247 or Scer\GAL4^c309 almost completely rescues the temperature preference behavior defects seen in Pka-C1^MB581/Pka-C1^X4 mutant.

(Hong et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Pka-C1^Scer\UAS.cKa

Pka-C1^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Kiger

(Kiger et al., 1999)

Secondary FlyBase IDs

References (12)

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