FlyBase Allele Report: Dmel\Rbp9[P2690]

General Information

Symbol

Dmel\Rbp9^P2690

Species

D. melanogaster

Name

FlyBase ID

FBal0097176

Feature type

allele

Associated gene

Dmel\Rbp9

Associated Insertion(s)

P{lacW}Rbp9^P2690

Carried in Construct

Also Known As

Rbp9^P[2690], Rbp9^P<up>2690</up>

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

loss of function allele

(Kim-Ha et al., 1999)

Mutagen

Delta2-3 transposase

(Kim-Ha et al., 1999)

Progenitor genotype

Rbp9^P1374

(Kim-Ha et al., 1999)

Associated Insertion(s)

P{lacW}Rbp9^P2690

Cytology

Description

An additional P{lacW} is inserted at nucleotide 7197, in the second coding exon. This maps to the first RNA binding domain.

(Kim-Ha et al., 1999)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:2,961,810..2,961,810

(Zaharieva et al., 2015, Kim et al., 2002, Kim-Ha et al., 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cyst cell of ovary

(Kim-Ha et al., 1999)

egg chamber

(Kim-Ha et al., 1999)

embryonic/larval neuromuscular junction

(Zaharieva et al., 2015)

germarium

(Kim-Ha et al., 1999)

NMJ bouton

(Zaharieva et al., 2015)

ovariole

(Kim-Ha et al., 1999)

subperineurial glial sheath | adult stage

(Kim et al., 2010)

Detailed Description

Statement

Reference

Neuromuscular junctions in Rbp9^P2690 mutant third instar larvae have a slightly increased number of synaptic boutons in comparison with controls.

Rbp9^P2690/Df(2L)ED206 adult flies do not exhibit any fusion of the mushroom body beta lobes.

Rbp9^P2690/Df(2L)ED206 mutant flies do not exhibit any defects in photoreceptors or central brain neuron maintenance.

Rbp9^P2690/Df(2L)ED206 mutant flies are short lived compared with controls.

(Zaharieva et al., 2015)

Mutant flies initially perform as well as wild-type flies in a climbing assay. However, over time, the mutant flies decline in performance more rapidly than the wild-type flies, such that at 13 days after eclosion, 50% pf the mutant flies have lost their climbing ability.

Mutants show a reduction in longevity compared to wild-type controls.

Mutant adults show an increase in the penetration of fluorescently labelled beads into the brain after injection into the abdomen, indicating a defect in the blood-brain barrier.

(Kim et al., 2010)

Mutant females show 0% fertility. Phenotype is very close to the null phenotype. Ovarioles of mutant females are filled with egg chambers with abnormal numbers of cystocytes that never develop beyond a stage 6 egg chamber. Germaria are enlarged. In a few cases the ovarioles are devoid of egg chambers. As the flies age, the underdeveloped egg chambers become filled with numerous small cells (the "tumerous bag" phenotype).

(Kim-Ha et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Rbp9^P2690/Df(2L)ED206 has short lived phenotype, non-enhanceable by Df(1)ED7165/Df(1)fne^Δ

(Zaharieva et al., 2015)

NOT Enhancer of

Statement

Reference

Rbp9^P2690/Df(2L)ED206 is a non-enhancer of abnormal neuroanatomy | embryonic stage phenotype of elav⁵/elav^ts1

(Zaharieva et al., 2015)

Rbp9^P2690 is a non-enhancer of abnormal neuroanatomy | embryonic stage phenotype of elav⁵

(Zaharieva et al., 2015)

Rbp9^P2690/Df(1)fne^Δ is a non-enhancer of abnormal neuroanatomy | embryonic stage phenotype of elav⁵

(Zaharieva et al., 2015)

Other

Statement

Reference

fne^KOZ2/Df(1)fne^Δ, Rbp9^P2690/Df(2L)ED206 has short lived phenotype

(Grzejda et al., 2022)

Df(1)fne^Δ, Rbp9^P2690 has viable phenotype

(Zaharieva et al., 2015)

Rbp9^P2690, Sxl^fP7B0 has partially lethal - majority die | maternal effect | female limited phenotype

(Zaharieva et al., 2015)

Rbp9^P2690, elav⁵/elav^ts1 has lethal - all die before end of pupal stage phenotype

(Zaharieva et al., 2015)

Df(1)fne^Δ, Rbp9^P2690, elav⁵/elav^ts1 has lethal - all die during embryonic stage phenotype

(Zaharieva et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Rbp9^P2690 has embryonic/larval neuromuscular junction phenotype, suppressible by Df(1)ED7165/Df(1)fne^Δ

(Zaharieva et al., 2015)

Rbp9^P2690 has NMJ bouton phenotype, suppressible by Df(1)ED7165/Df(1)fne^Δ

(Zaharieva et al., 2015)

NOT Enhancer of

Statement

Reference

Rbp9^P2690/Df(2L)ED206 is a non-enhancer of embryonic/larval neuromuscular junction phenotype of elav⁵/elav^ts1

(Zaharieva et al., 2015)

Rbp9^P2690 is a non-enhancer of female germline cell phenotype of Rbfox1^f02600/Rbfox1^e03440

(Tastan et al., 2010)

NOT Suppressor of

Statement

Reference

Rbp9^P2690 is a non-suppressor of female germline cell phenotype of Rbfox1^f02600/Rbfox1^e03440

(Tastan et al., 2010)

Other

Statement

Reference

fne^KOZ2/Df(1)fne^Δ, Rbp9^P2690/Df(2L)ED206 has adult brain phenotype

(Grzejda et al., 2022)

fne^KOZ2/Df(1)fne^Δ, Rbp9^P2690/Df(2L)ED206 has ribonucleoprotein granule | adult stage phenotype

(Grzejda et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Rbp9^P2690/Df(2L)ED206 does not enhance the neuromuscular junction phenotypes seen in elav⁵/elav^ts1 mutant third instar larvae.

The number of boutons in the neuromuscular junctions of Df(1)fne^Δ/Df(1)ED7165; Rbp9^P2690/Df(2L)ED206 double mutant third instar larvae is dramatically reduced. No defects in photoreceptor or central brain neuron maintenance are observed, except for occasional vacuolizations.

Rbp9^P2690/Df(2L)ED206 does not enhance the mushroom body beta lobe fusion seen in Df(1)fne^Δ/Df(1)ED7165 mutant adult flies.

The axon wiring defects seen in elav⁵ mutant embryos is not enhanced by Df(1)fne^Δ or Rbp9^P2690, or both.

Removing one copy of Rbp9^P2690 during oogenesis in combination with zygotic heterozygosity for Sxl^fP7B0 results in female lethality. No female lethality is seen in the reverse cross. This female lethality is suppressed when msl-3¹ is also zygotically removed.

(Zaharieva et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Rbp9^P2690 is rescued by Rbp9^+t17.1

(Kim et al., 2010)

Rbp9^P2690 is rescued by Rbp9^+t17.1

(Kim-Ha et al., 1999)

Comments

The age-dependent defect in climbing ability that is seen in Rbp9^P2690 flies is rescued by Rbp9^+t17.1.

(Kim et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

25778

w^*; P{lacW}Rbp9^P2690/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Rbp9^P2690

(Saha et al., 2023, Carrasco et al., 2020, Zaharieva et al., 2015)

Rbp9^P<up>2690</up>

(Kim et al., 2010, Kim et al., 2006)

Rbp9^P[2690]

(Kim et al., 2002, Lee et al., 2000, Kim-Ha et al., 1999)

rbp9²⁶⁹⁰

(Tastan et al., 2010)

rbp9^P2690

(Grzejda et al., 2022)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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