FB2024_03 , released June 25, 2024
Allele: Dmel\ShCD8.Mhc.GFP(S65T)
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General Information
Symbol
Dmel\ShCD8.Mhc.GFP(S65T)
Species
D. melanogaster
Name
FlyBase ID
FBal0097113
Feature type
allele
Associated gene
Dmel\Sh
Associated Insertion(s)
Carried in Construct
P{Mhc.CD8-GFP-Sh}
Also Known As
CD8-GFP-Sh, MHC-CD8-GFP-Sh, MHC-CD8-GFP-Shaker
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Zito et al., 1999)
Progenitor genotype
Carried in construct
P{Mhc.CD8-GFP-Sh}
Cytology
Description

The Mhc promoter drives expression of a coding region fusion. The fusion contains; the extracellular and transmembrane domains of Hsap\CD8A; amino acids 479-503 of the Sh B cytoplasmic domain and the GFP(S65T) fluorescent protein.

(Zito et al., 1999)
Allele components
Component
Use(s)
Regulatory region(s)
Mhc
Encoded product / tool
Sh
Tagged with
GFP(S65T)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter or direct label
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      muscle cell of ventral cervical muscle 25
      (Rivlin et al., 2004)
      muscle cell of ventral cervical muscle 26
      (Rivlin et al., 2004)
      muscle cell of prothoracic sternal anterior rotator muscle 31
      (Rivlin et al., 2004)
      muscle cell of prothoracic sternal adductor muscle 33
      (Rivlin et al., 2004)
      motor neuron
      (Rivlin et al., 2004)
      adult somatic muscle cell of abdomen | intense
      (Rivlin et al., 2004)
      somatic muscle cell of prothorax
      (Rivlin et al., 2004)
      day 1 of adulthood
      prothoracic ventral eclosion muscle cell | intense
      (Rivlin et al., 2004)
      day 3 of adulthood
      muscle cell of ventral cervical muscle 27 | faint
      (Rivlin et al., 2004)
      Additional Information
      Statement
      Reference

      In newly eclosed adults, GFP-tagged Sh protein is intensely localized to type I nerve terminals projecting onto the prothoracic ventral eclosions muscle, and weakly localized to terminals on the cervical and coxal muscles. Expression levels increase in the cervical and coxal synapses during the first five days after eclosion, with the exception of ventral cervical muscle 27, where expression remains faint. Strong expression is also observed at the type I terminals of the adult abdominal muscles. Extrasynaptic Sh immunoreactivity is also observed in all adult prothoracic muscles in a highly regular pattern resembling a reticular network.

      (Rivlin et al., 2004)
       
      Marker for
      embryonic/larval somatic muscle cell
      (Aberle et al., 2002)
      neuromuscular junction
      (Aberle et al., 2002)
      Reflects expression of
      Sh
      (Rivlin et al., 2004)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      There is no significant difference between numbers of type I boutons at muscle 12 between Sh<up>Mhc. T:Avic \GFP-S65T, T:Hsap \CD8A</up> larvae and wild type.

      (Zito et al., 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer

      Zito

      (Zito et al., 1999)
      Comments
      Comments

      Useful for simultaneously observing the muscles and synapses in live, developing larvae.

      (Zito et al., 1999)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      ShCD8.Mhc.GFP(S65T)
      ShMhc.T:Avic\GFP-S65T,T:Hsap\CD8A
      Name Synonyms
      Secondary FlyBase IDs
        References (22)