FlyBase Allele Report: Dmel\PlexA[UAS.cWa]

General Information

Symbol

Dmel\PlexA^UAS.cWa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Winberg

FlyBase ID

FBal0094368

Feature type

allele

Associated gene

Dmel\PlexA

Associated Insertion(s)

Carried in Construct

P{UAS-PlexA.W}

Also Known As

UAS-PlexA

Key Links

Transgenic product class

wild_type

(Winberg et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Winberg et al., 1998)

Mutagen

in vitro construct

(Winberg et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-PlexA.W}

Cytology

Description

PlexA is expressed under the control of UASt regulatory sequences.

(Winberg et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

PlexA

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Charcot-Marie-Tooth disease type 2D

modeled by GlyRS^P234KY-2.UAS

(Grice et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^elav.PLu

(Ayoob et al., 2004)

abnormal neuroanatomy, with Scer\GAL4^elav.PU

(Hung et al., 2013)

Phenotype Manifest In

central nervous system, with Scer\GAL4^elav.PU

(Hung et al., 2013)

fascicle | ectopic, with Scer\GAL4^elav.PLu

(Ayoob et al., 2004)

larval abdominal segmental nerve, with Scer\GAL4^elav.PLu

(Winberg et al., 1998)

larval intersegmental nerve, with Scer\GAL4^elav.PLu

(Winberg et al., 1998)

larval longitudinal connective, with Scer\GAL4^elav.PLu

(Winberg et al., 1998)

larval transverse nerve, with Scer\GAL4^elav.PLu

(Winberg et al., 1998)

Detailed Description

Statement

Reference

Scer\GAL4^elav.PU-mediated expression of PlexA^Scer\UAS.cWa results in CNS axon guidance defects - discontinuous, thin or missing CNS longitudinal connectives, and CNS axons abnormally crossing the midline.

(Hung et al., 2013)

Expression of plexA^Scer\UAS.cWa in embryos under the control of Scer\GAL4^elav.PLu causes thick bundles of axons to abnormally cross the midline.

(Ayoob et al., 2004)

Causes no mutant phenotype in the mushroom bodies when expression is driven by Scer\GAL4.

(Nicolai et al., 2003)

Embryos expressing plexA^Scer\UAS.cWa under the control of Scer\GAL4^elav.PLu show axon guidance defects in all parts of the motor projection and also in the central nervous system. The dorsal SNa branch splits prematurely into multiple projections in 66% of segments and the dorsal extension of the intersegmental nerve (ISN) defasciculates inappropriately in 25% of segments. The transverse nerve is stalled in 26% of segments, resulting in the 2 halves of the nerve failing to meet. ISNb axons often fail to reach their muscle targets, and the nerve displays a thickened clumped structure, with stalls at choice points 2 and 3. The Fas2-positive longitudinal tracts are less tightly fasciculated than normal and are not always continuous between segments. The outermost Fas2-positive fascicle defasciculates and extends away from the central nervous system.

(Winberg et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

PlexA^UAS.cWa, Scer\GAL4^elav.PLu has abnormal neuroanatomy phenotype, enhanceable by PlexA^UAS.cWa, Scer\GAL4^elav.PLu

(Ayoob et al., 2004)

Suppressed by

Statement

Reference

PlexA^UAS.cWa, Scer\GAL4^elav.PU has abnormal neuroanatomy phenotype, suppressible by SelR^UAS.cHa, Scer\GAL4^elav.PU

(Hung et al., 2013)

PlexA^UAS.cWa, Scer\GAL4^elav.PLu has abnormal neuroanatomy phenotype, suppressible | partially by Gyc76C^KG03723ex173

(Ayoob et al., 2004)

PlexA^UAS.cWa, Scer\GAL4^elav.PLu has abnormal neuroanatomy phenotype, suppressible | partially by Gyc76C^{D945A.UAS.Tag:MYC}, Scer\GAL4^elav.PLu

(Ayoob et al., 2004)

Enhancer of

Statement

Reference

PlexA^UAS.cWa, Scer\GAL4^elav.PLu is an enhancer of abnormal neuroanatomy phenotype of PlexA^UAS.cWa, Scer\GAL4^elav.PLu

(Ayoob et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

PlexA^UAS.cWa, Scer\GAL4^elav.PLu has fascicle | ectopic phenotype, enhanceable by PlexA^UAS.cWa, Scer\GAL4^elav.PLu

(Ayoob et al., 2004)

Suppressed by

Statement

Reference

PlexA^UAS.cWa, Scer\GAL4^elav.PU has central nervous system phenotype, suppressible by SelR^UAS.cHa, Scer\GAL4^elav.PU

(Hung et al., 2013)

PlexA^UAS.cWa, Scer\GAL4^elav.PLu has fascicle | ectopic phenotype, suppressible | partially by Gyc76C^KG03723ex173

(Ayoob et al., 2004)

PlexA^UAS.cWa, Scer\GAL4^elav.PLu has fascicle | ectopic phenotype, suppressible | partially by Gyc76C^{D945A.UAS.Tag:MYC}, Scer\GAL4^elav.PLu

(Ayoob et al., 2004)

Enhancer of

Statement

Reference

PlexA^UAS.cWa, Scer\GAL4^elav.PLu is an enhancer of fascicle | ectopic phenotype of PlexA^UAS.cWa, Scer\GAL4^elav.PLu

(Ayoob et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^elav.PU-mediated expression of SelR^Scer\UAS.cHa significantly rescues the axon guidance defects seen in SelR^Scer\UAS.cHa Scer\GAL4^elav.PU animals.

(Hung et al., 2013)

A Gyc76C^KG03723ex173 background partially suppresses the aberrant axon midline-crossing phenotype of embryos that express plexA^Scer\UAS.cWa under the control of Scer\GAL4^elav.PLu. In contrast, coexpression of Gyc76C^{Scer\UAS.T:Hsap\MYC} with plexA^Scer\UAS.cWa, under the control of Scer\GAL4^elav.PLu, enhances the axon guidance phenotype of plexA^Scer\UAS.cWa-expressing embryos, with many axon bundles crossing the midline. Coexpression of the dominant negative Gyc76C^{D945A.Scer\UAS.T:Hsap\MYC} transgene with plexA^Scer\UAS.cWa, under the control of Scer\GAL4^elav.PLu, results in a partial suppression of the plexA^Scer\UAS.cWa-induced axon guidance phenotype.

(Ayoob et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

PlexA^UAS.cWa/Scer\GAL4^elav.PLu partially rescues Df(4)C3

(Winberg et al., 1998)

Comments

The central nervous system and motor axon guidance defects of homozygous Df(4)C3 embryos are rescued by plexA^Scer\UAS.cWa expressed under the control of Scer\GAL4^elav.PLu.

(Winberg et al., 1998)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8479

w^*; P{UAS-PlexA.W}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

PlexA^Scer\UAS.cWa

PlexA^UAS.cWa

plexA^Scer\UAS.cWa

plexA^UAS.cWa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Winberg

(Winberg et al., 1998)

Secondary FlyBase IDs

References (6)

Report Sections

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