FlyBase Allele Report: Dmel\E(spl)m3-HLH[UAS.cLa]

General Information

Symbol

Dmel\E(spl)m3-HLH^UAS.cLa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Ligoxygakis

FlyBase ID

FBal0093289

Feature type

allele

Associated gene

Dmel\E(spl)m3-HLH

Associated Insertion(s)

Carried in Construct

P{UAS-E(spl)m3-HLH.L}

Also Known As

UAS-m3

Key Links

Transgenic product class

wild_type

(Ligoxygakis et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ligoxygakis et al., 1998)

Mutagen

in vitro construct

(Ligoxygakis et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-E(spl)m3-HLH.L}

Cytology

Description

UAS sequences regulatory sequences drive expression of E(spl)m3-HLH.

(Ligoxygakis et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

E(spl)m3-HLH

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of pupal stage, with Scer\GAL4^32B

(Ligoxygakis et al., 1999)

some die during pupal stage, with Scer\GAL4^32B

(Ligoxygakis et al., 1999)

visible, with Scer\GAL4^32B

(Ligoxygakis et al., 1999)

visible, with Scer\GAL4^455.2

(Ligoxygakis et al., 1999)

visible, with Scer\GAL4^ap-md544

(Ligoxygakis et al., 1999)

visible, with Scer\GAL4^h-1J3

(Ligoxygakis et al., 1999)

Phenotype Manifest In

scutellar bristle, with Scer\GAL4^455.2

(Ligoxygakis et al., 1999)

scutum & macrochaeta, with Scer\GAL4^ap-md544

(Ligoxygakis et al., 1999)

scutum & macrochaeta, with Scer\GAL4^h-1J3

(Ligoxygakis et al., 1999)

scutum & microchaeta, with Scer\GAL4^32B

(Ligoxygakis et al., 1999)

scutum & microchaeta, with Scer\GAL4^ap-md544

(Ligoxygakis et al., 1999)

Detailed Description

Statement

Reference

Animals expressing HLHm3^Scer\UAS.cLa under the control of Scer\GAL4^32B show pupal lethality. Loss of notum microchaetae is seen. Flies expressing HLHm3^Scer\UAS.cLa under the control of Scer\GAL4^ap-md544 show complete loss of notum microchaetae and macrochaetae, except at the very anterior edge of the notum. Flies expressing HLHm3^Scer\UAS.cLa under the control of Scer\GAL4^455.2 show severe loss of scutellar bristles. Flies expressing HLHm3^Scer\UAS.cLa under the control of Scer\GAL4^h-1J3 show loss of notum macrochaetae.

(Ligoxygakis et al., 1999)

Flies expressing one copy of HLHm3^Scer\UAS.cLa under the control of one copy of Scer\GAL4^h-H10 are phenotypically wild-type.

(Ligoxygakis et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

The relative ability of UAS constructs of the E(spl) complex to induce loss of notum microchaetae when expressed under the control of Scer\GAL4^32B is (ranked according to severity of phenotype): E(spl)^Scer\UAS.cNa > HLHm7^{Scer\UAS.cdCa} >> HLHmγ^Scer\UAS.cLa = HLHmβ^{Scer\UAS.cdCa} = HLHm3^Scer\UAS.cLa > HLHmδ^{Scer\UAS.cdCa}. The relative ability of UAS constructs of the E(spl) complex to induce loss of notum macrochaetae when expressed under the control of Scer\GAL4^h-1J3 is (ranked according to severity of phenotype): E(spl)^Scer\UAS.cNa > HLHm7^{Scer\UAS.cdCa} > HLHmβ^{Scer\UAS.cdCa} > HLHmδ^{Scer\UAS.cdCa} > HLHmγ^Scer\UAS.cLa = HLHm3^Scer\UAS.cLa.

(Ligoxygakis et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

E(spl)m3-HLH^Scer\UAS.cLa

E(spl)m3-HLH^UAS.cLa

HLHm3^Scer\UAS.cLa

HLHm3^UAS.cLa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Ligoxygakis

(Ligoxygakis et al., 1998)

Secondary FlyBase IDs

References (5)

Report Sections

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