Expression of Rat\CamKII-I^Ala.Scer\UAS under the control of Scer\GAL4^how-24B has no effect on the amplitude of evoked activity events visualized by the Avic\GFP^{SynapGCaMP6f.Mhc} sensor neither does it affect density of active synapses or variability in the release probability at either motor neuron Is or Ib input but it increases both the single-synapse release probability and quantal density per stimulus at Ib synapses while neither of these parameters is affected at Is synapses.

The expression of Rat\CamKII-I^Ala.Scer\UAS under the control of Scer\GAL4^Toll-6-D42 leads to significant decreases in both the axonal length and the number of active zones at the larval neuromuscular junction, as compared to controls.

Expression of Rat\CamKII-I^Ala.Scer\UAS in the RP2 motor neurons (using the Scer\GAL4^eve.RRK driver to drive expression of Scer\FLP1^Scer\UAS.cUa which then induces clones of cells expressing Scer\GAL4^Act.PU) results in a significant decrease in the number of dendritic branches and total dendrite length compared to controls. The overall pattern of branching is changed such that there are a greater number of branches closer to the neuronal soma.

Expression of Rat\CamKII-I^Ala.Scer\UAS under the control of Scer\GAL4^how-24B has no effect on the area of the presynaptic terminal at the larval neuromuscular junction.

At 1.5mM extracellular Ca[2+], the mean amplitude of the excitatory junctional potential (EJP) at the neuromuscular junction is increased compared to control larvae in larvae expressing Rat\CamKII-I^Ala.Scer\UAS under the control of Scer\GAL4^how-24B. However, at 0.75mM extracellular Ca[2+], the mean EJP at the mutant neuromuscular junction is not significantly different from that of controls. The mean amplitude of spontaneous miniature EJPs at the mutant neuromuscular junction is unchanged compared to controls. The quantal content is significantly elevated in the mutant larvae at 1.5mM extracellular Ca[2+].

Expression of Rat\CamKII-I^Ala.Scer\UAS (either alone or under the control of Scer\GAL4^hs.2sev) has little or no effect on free-running circadian period.

In neuromuscular junctions of Rat\CamKII-I^Ala.Scer\UAS larvae carrying Scer\GAL4^Mef2.PR or Scer\GAL4^Mhc.PW, but not Scer\GAL4^elav.PLu, excitatory postsynaptic potential (EPSP) amplitude is increased by 20% compared to wild-type (p < 0.001) resulting in a 20% increase (p < 0.005) in quantal content. However, the amplitude and frequency and kinetics of mini-EPSPs are not significantly different from wild-type and neither are membrane potential and input resistance of the muscles. (Data from electrophysiological recordings made from muscle 6 in segment A3 of wandering 3rd instar larvae). The overall numbers of boutons and the numbers of boutons per muscle surface area in Rat\CamKII-I^Ala.Scer\UAS larvae carrying Scer\GAL4^Mhc.PW or Scer\GAL4^elav.PLu are not significantly different from wild-type. (Data from neuromuscular junctions of muscles 6 and 7 in segment A3 of wandering 3rd instar larvae). The overall structure of these bouton, the subsynaptic reticulum structure and the number of clear synaptic vesicles appear to be qualitatively wild-type. However, boutons in Rat\CamKII-I^Ala.Scer\UAS; Scer\GAL4^Mhc.PW but not Rat\CamKII-I^Ala.Scer\UAS; Scer\GAL4^elav.PLu larvae, display an unusual invagination of the presynaptic membrane at active zones and have a 60% increase in the number of t-bars per active zone (p < 0.001).

Flies expressing Rat\CamKII-I^Ala.Scer\UAS under the control of one of Scer\GAL4^29BD, Scer\GAL4^30Y, Scer\GAL4^Tab2-201Y, Scer\GAL4^OK348, Scer\GAL4^40B, Scer\GAL4^MJ162A, Scer\GAL4^MJ146, Scer\GAL4^MJ63 or Scer\GAL4^MJ94 show normal locomotor activity. Expression of Rat\CamKII-I^Ala.Scer\UAS under the control of Scer\GAL4^Tab2-201Y or Scer\GAL4^MJ94 results in males with an abnormal courtship response during training with a mated female (wild-type males show a decrement in courtship during training, with the ratio of the final courtship index/initial courtship index being approximately 0.5. This ratio is significantly increased in the mutant flies). This phenotype is seen both in the presence and absence of visual input. Expression of Rat\CamKII-I^Ala.Scer\UAS under the control of Scer\GAL4^OK348, Scer\GAL4^MJ63 or Scer\GAL4^40B results in males with an abnormal courtship response during training with a mated female (wild-type males show a decrement in courtship during training, with the ratio of the final courtship index/initial courtship index being approximately 0.5. This ratio is significantly increased in the mutant flies). This phenotype is only seen in the presence of visual input. Expression of Rat\CamKII-I^Ala.Scer\UAS under the control of Scer\GAL4^MJ162A or Scer\GAL4^MJ146 results in males with an abnormal courtship response during training with a mated female (wild-type males show a decrement in courtship during training, with the ratio of the final courtship index/initial courtship index being approximately 0.5. This ratio is significantly increased in the mutant flies). This phenotype is only seen in the absence of visual input. Expression of Rat\CamKII-I^Ala.Scer\UAS under the control of one of Scer\GAL4^29BD, Scer\GAL4^30Y, Scer\GAL4^Tab2-201Y, Scer\GAL4^OK348, Scer\GAL4^40B, Scer\GAL4^MJ162A, Scer\GAL4^MJ146, Scer\GAL4^MJ63 or Scer\GAL4^MJ94 does not disrupt memory formation in males trained in a courtship conditioning assay under white light. However, in the absence of visual input (red light), expression of Rat\CamKII-I^Ala.Scer\UAS under the control of one of Scer\GAL4^29BD, Scer\GAL4^30Y, Scer\GAL4^Tab2-201Y, Scer\GAL4^OK348 or Scer\GAL4^MJ63 blocks formation of memory in this assay.

Expression of Rat\CamKII-I^Ala.Scer\UAS under the control of Scer\GAL4^unspecified at both pre- and postsynaptic sites at the larval neuromuscular junction does not result in gross morphological changes. However, there is a significant alteration in bouton ultrastructure; the SSR appears more extensive than wild type. The number of active zones in type I boutons is similar to wild type.

Flies expressing Rat\CamKII-I^Ala.Scer\UAS under the control of Scer\GAL4^c362 have a decreased reflex response compared to wild-type flies in an assay in which the resistance response from the tibial extensor motor neurons in response to flexing the femorotibial joint is measured. The initial reflex response is only 33% of the response in control flies. Habituation of this reflex response is blocked. The axon projections of the femoral chordotonal organ sensory neurons are normal in flies expressing Rat\CamKII-I^Ala.Scer\UAS under the control of Scer\GAL4^c362.

Scer\GAL4^MJ85b-mediated expression in males does not cause a block in memory, even in the apparent absence of learning, in courtship behaviour assays.

Rat\CamKII-I^Ala.UAS, Scer\FLP1^UAS.cUa, Scer\GAL4^Act.PU, Scer\GAL4^eve.RRK has abnormal neuroanatomy | somatic clone phenotype, suppressible | partially | somatic clone by Adf1^{S64D.S184D.UAS.Tag:FLAG}, Scer\FLP1^UAS.cUa, Scer\GAL4^Act.PU, Scer\GAL4^eve.RRK

Rat\CamKII-I^Ala.UAS, Scer\GAL4^Mhc.PW has abnormal neurophysiology phenotype, suppressible by CaMKII^T287D.UAS, Scer\GAL4^Mhc.PW

Rat\CamKII-I^Ala.UAS, Scer\FLP1^UAS.cUa, Scer\GAL4^Act.PU, Scer\GAL4^eve.RRK has dendrite | somatic clone phenotype, suppressible | partially | somatic clone by Adf1^{S64D.S184D.UAS.Tag:FLAG}, Scer\FLP1^UAS.cUa, Scer\GAL4^Act.PU, Scer\GAL4^eve.RRK

Rat\CamKII-I^Ala.UAS, Scer\FLP1^UAS.cUa, Scer\GAL4^Act.PU, Scer\GAL4^eve.RRK has larval RP2 motor neuron | somatic clone phenotype, suppressible | partially | somatic clone by Adf1^{S64D.S184D.UAS.Tag:FLAG}, Scer\FLP1^UAS.cUa, Scer\GAL4^Act.PU, Scer\GAL4^eve.RRK