FB2024_03 , released June 25, 2024
Allele: Ecol\lacZUbx.PdC
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General Information
Symbol
Ecol\lacZUbx.PdC
Species
E. coli
Name
Ultrabithorax promoter construct of de Celis
FlyBase ID
FBal0065771
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
Carried in Construct
P{GAL4-lacZ-Ubx(-FRT).dC}
Also Known As
Ubx-Gal4, UbxGal4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
FLPase
(Moreno et al., 2002, Azpiazu and Morata, 2000, Baonza et al., 2000, de Celis and Bray, 2000, de Celis and Bray, 1997)
Progenitor genotype
Ecol\lacZFRT.Ubx
(Moreno et al., 2002, Azpiazu and Morata, 2000, Baonza et al., 2000, de Celis and Bray, 2000, de Celis and Bray, 1997)
Carried in construct
P{GAL4-lacZ-Ubx(-FRT).dC}
Cytology
Description

The FRT cassette has been excised, allowing the Ubx promoter to drive expression of a coding cassette composed of GAL4 and lacZ (the two coding regions are separated by an internal ribosome entry site).

(de Celis and Bray, 1997)
Allele components
Component
Use(s)
Regulatory region(s)
Ubx
Encoded product / tool
lacZ
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      larval stage
      wing disc
      (de Celis and Bray, 1997)
      third instar larval stage
      wing disc
      (de Celis and Bray, 1997)
      adult stage
      adult abdomen
      (Lawrence et al., 1999)
      cuticle of adult abdomen
      (Lawrence et al., 1999)
      Additional Information
      Statement
      Reference

      Used for clonal analysis in wing disc; EcollacZ serves as marker for ScerGAL4-expressing cells.

      (de Celis and Bray, 1997)
       
      Marker for
      Reflects expression of
      Ubx
      (de Celis and Bray, 1997)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Exists in clones, not reported as germ-line transmitted. Scer\GAL4 and Ecol\lacZ are expressed from a dicistronic transcript in this construct.

      (de Celis and Bray, 1997)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Ecol\lacZUbx.PdC
      lacZUbx.PdC
      Name Synonyms
      Ultrabithorax promoter construct of de Celis
      (de Celis and Bray, 1997)
      Secondary FlyBase IDs
        References (7)