FlyBase Allele Report: Mmus\Nos2[hs.PK]

General Information

Symbol

Mmus\Nos2^hs.PK

Species

M. musculus

Name

heat shock construct of Kuzin

FlyBase ID

FBal0058515

Feature type

allele

Associated gene

Mmus\Nos2

Associated Insertion(s)

Carried in Construct

P{hs-mNOS}

Transgenic product class

cDNA

(Kuzin et al., 1996)

wild_type

(Kuzin et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Kuzin et al., 1996)

wild_type

(Kuzin et al., 1996)

Mutagen

in vitro construct

(Kuzin et al., 1996)

Progenitor genotype

Carried in construct

P{hs-mNOS}

Cytology

Description

Expression of a 4100bp NotI cDNA fragment containing the entire Mmus\Nos2 gene is controlled by an Hsp70 promoter.

(Kuzin et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Mmus\Nos2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior

(Wingrove and O'Farrell, 1999)

abnormal size | adult stage

(Kuzin et al., 1996)

abnormal size | third instar larval stage | temperature conditional

(Jaszczak et al., 2016)

decreased occurrence of cell division | larval stage

(Kuzin et al., 1996)

Phenotype Manifest In

embryonic/larval tracheal system

(Wingrove and O'Farrell, 1999)

eye disc | third instar larval stage | temperature conditional

(Jaszczak et al., 2016)

pretarsus

(Kuzin et al., 1996)

tarsal segment 3

(Kuzin et al., 1996)

tarsal segment 4

(Kuzin et al., 1996)

Detailed Description

Statement

Reference

Ectopic heat-shock induced expression of Mmus\Nos2^hs.PK in the absence of any tissue damage inhibits eye disc growth in third instar larvae compared to age-matched controls.

(Jaszczak et al., 2016)

Induced expression leads to hypersensitivity to 10% oxygen hypoxia, seen as a high proportion of larvae leaving the yeast in the second phase of behavioural response. Induction of Mmus\Nos2^hs.PK increases the number and length of terminal branches in the tracheal system.

(Wingrove and O'Farrell, 1999)

Heat induced expression at larval stages causes suppression of DNA synthesis and cell division. Heat induced expression within 1 hour after pupariation results in a reduction in the size of the limbs, distal segments of the legs are affected most frequently and to the greatest degree.

(Kuzin et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Mmus\Nos2^hs.PK has abnormal size | third instar larval stage | temperature conditional phenotype, non-suppressible by Scer\GAL4^elav.PU/Lgr3^GL01056

(Jaszczak et al., 2016)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Mmus\Nos2^hs.PK has eye disc | third instar larval stage | temperature conditional phenotype, non-suppressible by Scer\GAL4^elav.PU/Lgr3^GL01056

(Jaszczak et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The inhibition of eye disc growth (decreased size) in the absence of tissue damage in third instar larvae resulting from heat-shock induced ectopic expression of Mmus\Nos2^hs.PK cannot be suppressed by Scer\GAL4^elav.PU-driven expression of Lgr3^GL01056.

(Jaszczak et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Calcium independent form of nitric oxide synthase that is capable of efficient constitutive nitric oxide production.

(Kuzin et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Mmus\Nos2^hs.PK

Mus\NOS2^hs.PK

Mus\Nos2^hs.PK

Name Synonyms

heat shock construct of Kuzin

(Kuzin et al., 1996)

Secondary FlyBase IDs

References (3)

Report Sections

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