FB2024_03 , released June 25, 2024
Allele: Dmel\ShB.hs
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General Information
Symbol
Dmel\ShB.hs
Species
D. melanogaster
Name
FlyBase ID
FBal0049032
Feature type
allele
Associated gene
Dmel\Sh
Associated Insertion(s)
Carried in Construct
P{hsp70-ShB}
Key Links
Transgenic product class
cDNA
(Gisselmann et al., 1989)
wild_type
(Gisselmann et al., 1989)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Gisselmann et al., 1989)
wild_type
(Gisselmann et al., 1989)
Mutagen
in vitro construct
(Gisselmann et al., 1989, Zagotta et al., 1989)
Progenitor genotype
Carried in construct
P{hsp70-ShB}
Cytology
Description

Expression of Sh B cDNA is driven by an Hsp70 promoter.

(Gisselmann et al., 1989)

Expression of ShB cDNA is under the control of an Hsp70 promoter region from -291 to +62 (including the TATA box and transcription start site).

(Zagotta et al., 1989)
Allele components
Component
Use(s)
Regulatory region(s)
Hsp70
Encoded product / tool
Sh
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Encodes a functional A-type channel (according to expression studies in Xenopus oocytes, FBrf0048790). Heat shock does not induce a vigorous leg shaking phenotype.

      (Gisselmann et al., 1989)

      ShB channels are expressed in primary cultured cells from transformant embryos. The current, mean open time and median first latency parameters are within the range of the parameters for both the native channel and ShB channel expressed in Xenopus oocytes. Similar to expression in oocytes the transformant channels do not exhibit a pulse-dependent rundown phenomenon (indicating rapid recovery from inactivation) and similar to the native channel they are insensitive to charybdotoxin (CTX).

      (Zagotta et al., 1989)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      ShB.hs
      Name Synonyms
      Secondary FlyBase IDs
        References (2)