Amino acid replacement: P446L.
Nucleotide substitution: C4114T.
C20737522T
C4114T
P446L | Fs(2)Ket-PA; P446L | Fs(2)Ket-PB; P446L | Fs(2)Ket-PC; P446L | Fs(2)Ket-PD; P446L | Fs(2)Ket-PE
P446L
Heterozygous females deposit normal numbers of normal looking eggs. The eggs are normally fertilised as revealed by the presence of a sperm tail in the egg cytoplasm. As in wild type, the nuclei are well contoured in newly deposited eggs, suggesting compact nuclei. The four haploid nuclei appear compact in unfertilised eggs laid by virgin heterozygous females. A bundle of microtubules is seen between the two inner haploid nuclei and is most likely the central spindle pole body that persists following the second meiotic division (this phenotype is never seen in wild-type eggs). Severe defects appear 6-7 minutes after fertilisation when the female and male pronuclei become juxtaposed; the male and female pronuclei are poorly contoured suggesting nuclear envelope defects. Generally, disorganised masses of microtubules (MTs) form instead of the gonomeric spindle. The MT mass appears as a prominent sperm aster and persists for several minutes. The centrosome replicates, however, the daughter centrosomes cannot separate. The chromosomes fail to segregate and disintegrate in minutes. The centrosomes may replicate 2 or 3 times but instead of separating they organise rudimentary asters of MTs along with a general decay of the egg cytoplasm. Dp(2;Y)G does not reduce the sterility of Fs(2)Ket3/+ females.
Heterozygous females are fully viable and fertile. Mutant phenotype may be 'cured' by increasing the normal gene product ratio.
Eggs laid by heterozygous females show meiotic defects; the polar body and oocyte nuclei are missing or abnormal.
When Fs(2)Ket3/+ females carrying two copies of Fs(2)Ket+t22 are mated to males carrying one copy of Fs(2)Ket+t22 a few offspring are produced.
Analysis of germ-line chimeras shows that this mutation is germ-line- dependent.