FB2024_03 , released June 25, 2024
Allele: Dmel\Fs(2)Ket2
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General Information
Symbol
Dmel\Fs(2)Ket2
Species
D. melanogaster
Name
FlyBase ID
FBal0048751
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
KetelD2
Key Links
Nature of the Allele
Progenitor genotype
Cytology
Description

Amino acid replacement: P446L.

Nucleotide substitution: C4114T.

Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Heterozygous females deposit normal numbers of normal looking eggs. The eggs are normally fertilised as revealed by the presence of a sperm tail in the egg cytoplasm. As in wild type, the nuclei are well contoured in newly deposited eggs, suggesting compact nuclei. The four haploid nuclei appear compact in unfertilised eggs laid by virgin heterozygous females. A bundle of microtubules is seen between the two inner haploid nuclei and is most likely the central spindle pole body that persists following the second meiotic division (this phenotype is never seen in wild-type eggs). Severe defects appear 6-7 minutes after fertilisation when the female and male pronuclei become juxtaposed; the male and female pronuclei are poorly contoured suggesting nuclear envelope defects. Generally, disorganised masses of microtubules (MTs) form instead of the gonomeric spindle. The MT mass appears as a prominent sperm aster and persists for several minutes. The centrosome replicates, however, the daughter centrosomes cannot separate. The chromosomes fail to segregate and disintegrate in minutes. The centrosomes may replicate 2 or 3 times but instead of separating they organise rudimentary asters of MTs along with a general decay of the egg cytoplasm. About 1% of eggs derived from Fs(2)Ket2 Dp(2;Y)G females turn brown, indicating the progression of embryogenesis to the stage of cuticle formation. In addition a small number of adult offspring are produced.

Heterozygous females are fully viable and fertile. Mutant phenotype may be 'cured' by increasing the normal gene product ratio.

About 50% of the eggs from heterozygous females appear unfertilised. The remainder can undergo cleavage divisions up to the syncytial blastoderm stage.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The dominant female sterility of Fs(2)Ket2 is slightly rescued by Fs(2)Ket+t22 or Fs(2)KetIII; approximately 1% of eggs laid by females progress to the stage of embryonic cuticle formation.

The dominant female sterility of Fs(2)Ket2 is slightly rescued by Fs(2)Ket+t22; cuticle develops in about 1% of eggs and a few offspring are produced.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Analysis of germ-line chimeras shows that this mutation is germ-line- dependent.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (6)