FlyBase Allele Report: Ecol\lacZ[Act5C.PS.Tag:NLS(Unk)]

General Information

Symbol

Ecol\lacZ^{Act5C.PS.Tag:NLS(Unk)}

Species

E. coli

Name

Actin 5C promoter construct of Struhl

FlyBase ID

FBal0043835

Feature type

allele

Associated gene

Ecol\lacZ

Associated Insertion(s)

Carried in Construct

P{Act5C(-FRT)lacZ.nls1}

Transgenic product class

Mutagen

FLPase

Nature of the Allele

Transgenic product class

Mutagen

FLPase

(Lee et al., 2006, Gorfinkiel et al., 2003, Vegh and Basler, 2003, Rozowski and Akam, 2002, Rangarajan et al., 1999, Weigmann and Cohen, 1999, Campbell and Tomlinson, 1998, Park et al., 1998, Woodhouse et al., 1998, Okabe and Okano, 1997, Reddy et al., 1997, Brewster and Bodmer, 1995, Struhl and Basler, 1993)

Progenitor genotype

Ecol\lacZ^{FRT.Act5C.cBa.Tag:NLS(Unk)}

(Lee et al., 2006, de la Cova et al., 2004, Gorfinkiel et al., 2003, Vegh and Basler, 2003, Rozowski and Akam, 2002, Rangarajan et al., 1999, Weigmann and Cohen, 1999, Campbell and Tomlinson, 1998, Park et al., 1998, Woodhouse et al., 1998, Okabe and Okano, 1997, Reddy et al., 1997, Brewster and Bodmer, 1995, Struhl and Basler, 1993)

Carried in construct

P{Act5C(-FRT)lacZ.nls1}

Cytology

Description

FRT cassette has been excised, allowing expression of the nuclear localized lacZ reporter under the control of the Act5C promoter.

(Struhl and Basler, 1993)

Allele components

Component

Use(s)

Regulatory region(s)

Act5C

Act5C_promoter

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(Unk)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

wing disc

(Struhl and Basler, 1993)

imaginal disc

(Struhl and Basler, 1993)

Additional Information

Statement

Reference

Present in clones in animals carrying the progenitor transposon P{Act5C(FRT.polyA)lacZ.nls1} and exposed to a source of ScerFLP1.

(Struhl and Basler, 1993)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Exists in clones, not reported as being germ-line transmitted.

(Struhl and Basler, 1993)

Exists in clones, not reported as being germ-line transmitted. Used in cell-lineage analysis of the chordotonal precursor cells.

(Okabe and Okano, 1997)

Exists in clones, not reported as being germ-line transmitted. Used in clonal analysis of olfactory sense organs.

(Reddy et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Ecol\lacZ^{Act5C.PS.T:Uuuu\nls-Unk}

Ecol\lacZ^{Act5C.PS.T:Zzzz\nls-KKKRKV}

Ecol\lacZ^{Act5C.PS.T:Zzzz\nls1}

Ecol\lacZ^{Act5C.PS.Tag:NLS(Unk)}

lacZ^{Act5C.PS.T:nls1}

Name Synonyms

Actin 5C promoter construct of Struhl

(Struhl and Basler, 1993)

Secondary FlyBase IDs

References (33)

Report Sections

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