lacZ gene expression is driven by module D of the Dfd epidermal autoregulatory element (Dfd EAE) fused to an Hsp70 minimal promoter.
Dfd promoter sequences mapping between 5.4 to 4.5 kb upstream of the Dfd transcription start site are fused to a heat shock gene basal promoter, the lacZ coding sequence and an heat shock gene polyadenylation site.