P{π25.1} has been introduced into the germline of D.simulans and the invasion kinetics of P-elements have been studied in 7 independent lines over 60 generations. Some of the main phenotypic and molecular characteristics of P-M hybrid dysgenesis have been observed, for example, gonadal dysgenesis (GD sterility), chromosome rearrangements, and the occurrence of degenerate P-elements. At least 4 lines reached a steady state with complete or nearly complete P-element regulation, but with a moderate number of P-elements (10-24 per haploid genome) and P activity (10-35% GD sterility).
The P{π25.1} element containing P\T+t2.9 and the H{FL1} element containing hobo\T+t2.96 have been independently introduced into the Hikone strain (an ME strain lacking hobo and P-element sequences), to compare the dynamics of P-element and hobo transposition in the same genetic background.
Ten lines of D.simulans have been investigated with respect to P activity, P susceptibility and the number and structure of their P-element copies, eight years after transformation with P{π25.1}. All the lines have reached a steady state. They exhibit varying levels of P activity (from 0 to 96% GD sterility) and are not P-susceptible (with the exception of one line). In contrast to P-element behaviour in D.melanogaster, no relationship is found between the molecular pattern of P-element copies in a line and its ability to induce or repress P expression in D.simulans, peculiar P-element derivatives are observed in D.simulans and the average number of P-element copies per genome is half that of D.melanogaster.
Used as a transposase source.
P{π25.1} has been introduced into the germline of D.simulans and the invasion kinetics of P-elements have been studied in 7 independent lines over 60 generations. Some of the main phenotypic and molecular characteristics of P-M hybrid dysgenesis have been observed, for example, gonadal dysgenesis (GD sterility), chromosome rearrangements, and the occurrence of degenerate P-elements. At least 4 lines reached a steady state with complete or nearly complete P-element regulation, but with a moderate number of P-elements (10-24 per haploid genome) and P activity (10-35% GD sterility).
The P{π25.1} element containing P\T+t2.9 and the H{FL1} element containing hobo\T+t2.96 have been independently introduced into the Hikone strain (an ME strain lacking hobo and P-element sequences), to compare the dynamics of P-element and hobo transposition in the same genetic background.
Ten lines of D.simulans have been investigated with respect to P activity, P susceptibility and the number and structure of their P-element copies, eight years after transformation with P{π25.1}. All the lines have reached a steady state. They exhibit varying levels of P activity (from 0 to 96% GD sterility) and are not P-susceptible (with the exception of one line). In contrast to P-element behaviour in D.melanogaster, no relationship is found between the molecular pattern of P-element copies in a line and its ability to induce or repress P expression in D.simulans, peculiar P-element derivatives are observed in D.simulans and the average number of P-element copies per genome is half that of D.melanogaster.