FB2024_03 , released June 25, 2024
Allele: Ecol\lacZsim.7.8
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General Information
Symbol
Ecol\lacZsim.7.8
Species
E. coli
Name
FlyBase ID
FBal0035613
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
Carried in Construct
P{7.8sim-lacZ}
Also Known As
sim-lacZ
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Menne and Klambt, 1994, Crews et al., 1992, Nambu et al., 1991, Nambu et al., 1990)
Progenitor genotype
Carried in construct
P{7.8sim-lacZ}
Cytology
Description

A 7.8kb genomic fragment from the sim locus that includes a set of regulatory elements is fused upstream of the lacZ coding sequence. The sim fragment includes part of the sim coding sequence and thus expression is predicted to result in a fusion protein that contains the N-terminal 482 amino acids of sim fused to lacZ starting at amino acid 7. The sim coding sequence also includes a nuclear localisation signal.

(Nambu et al., 1990)
Allele components
Component
Use(s)
Encoded product / tool
lacZ
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage 8 -- 17
      (Nambu et al., 1990)
      embryonic stage 10
      posterior midgut primordium
      (Nambu et al., 1990)
      stomodeum | restricted
      (Nambu et al., 1990)
      proctodeum
      (Nambu et al., 1990)
      embryonic stage 10 -- 17
      ventral midline of embryo
      (Nambu et al., 1990)
      embryonic stage 13 -- 17
      larval ventral nerve cord | restricted
      (Nambu et al., 1990)
      Additional Information
      Statement
      Reference

      At stage 15, expression observed in midline glia and in some midline neurons; at this stage, wild-type sim expressed in glia only.

      (Nambu et al., 1990)
       
      Marker for
      ventral midline of embryo
      (Nambu et al., 1990)
      Reflects expression of
      sim
      (Nambu et al., 1990)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Comments
      Comments

      Construct has a weak sim rescuing activity.

      (Nambu et al., 1991, Nambu et al., 1990)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Ecol\lacZsim.7.8
      lacZsim.7.8
      Name Synonyms
      Secondary FlyBase IDs
        References (7)