FlyBase Allele Report: Dmel\pnut[XP]

General Information

Symbol

Dmel\pnut^XP

Species

D. melanogaster

Name

FlyBase ID

FBal0035461

Feature type

allele

Associated gene

Dmel\pnut

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Neufeld and Rubin, 1994)

Progenitor genotype

pnut^rN498

(Neufeld and Rubin, 1994)

Cytology

Description

Deletion of at least 17kb that lacks nearly all of the pnut coding region.

(Neufeld and Rubin, 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Kaltenbach et al., 2007)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

pnut^XP heterozygous adults show increased retinal electrical response to green light (increased amplitude), compared to controls.

(Kumari et al., 2022)

pnut^XP mutant larvae lack imaginal discs and show polyploidy metaphases in neural ganglia. pnut^XP mutant larvae also carrying pnut^{K155A.D206A.K288A.pnut.T:Zzzz\FLAG} show an indistinguishable phenotype from that of pnut^XP single mutants.

(Akhmetova et al., 2015)

pnut^XP flies in which pnut^hs.PN is expressed using daily heat shocks during larval and pupal development survive to adulthood. Female flies efficiently produce eggs. However, in most crosses of these females to either wild-type or pnut^rN498/+ males, all of the embryos die without hatching, even when heat shocks are continued for 1 to 5 days after eclosion of the adult flies. Events prior to gastrulation appear to occur normally in embryos derived from a cross of pnut^XP females carrying pnut^hs.PN (and rescued to adulthood by daily heat shock) with either wild-type or pnut^XP/+ males; a layer of cleared cytoplasm forms at the cortex and cellularisation begins and proceeds evenly over the entire embryo at a normal speed and depth, although the cellularisation front is typically somewhat less well defined than in wild-type embryos. Global defects are generally not seen until the onset of gastrulation, when an ectopic fold is often seen anterior to the cephalic furrow. In addition, the posterior midgut plate often migrates to the left or right of the embryo instead of dorsally.

(Adam et al., 2000)

Dissection of the third instar larvae revealed severely reduced imaginal discs. Mutant brains contain a large number of polyploid cells. Most often these cells are tetraploid, but a poidy of 8 or more has been observed. Nucler to cytoplasm ratio measurements indicated a failure in cytokinesis. Large multinucleate cells were found in all imaginal tissues examined, with most severe effects in the lymph glands and imaginal rings of the salivary glands, in which nearly all cells contain multiple nuclei.

(Neufeld and Rubin, 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

pnut^XP has abnormal neurophysiology | dominant | adult stage phenotype, suppressible by rdgB⁹

(Kumari et al., 2022)

pnut^XP has abnormal neurophysiology | dominant | adult stage phenotype, suppressible by PIP5K59B^{RNAi.UAS.L.cKa}/Scer\GAL4^ninaE.PT

(Kumari et al., 2022)

Enhancer of

Statement

Reference

pnut^XP is an enhancer of abnormal cell polarity phenotype of S^48-5

(Gaengel and Mlodzik, 2003)

Suppressor of

Statement

Reference

pnut[+]/pnut^XP is a suppressor of abnormal neurophysiology | adult stage phenotype of rdgB⁹

(Kumari et al., 2022)

pnut^XP is a suppressor of visible phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Kaltenbach et al., 2007)

pnut[+]/pnut^XP is a suppressor of visible phenotype of rpr^GMR.PW

(Gottfried et al., 2004)

pnut[+]/pnut^XP is a suppressor of visible phenotype of hid^GMR.PG

(Gottfried et al., 2004)

pnut[+]/pnut^XP is a suppressor of visible phenotype of grim^GMR.PC

(Gottfried et al., 2004)

Phenotype Manifest In

Suppressed by

Statement

Reference

pnut^XP has retina phenotype, suppressible by rdgB⁹

(Kumari et al., 2022)

Enhancer of

Statement

Reference

pnut^XP is an enhancer of ommatidium phenotype of S^48-5

(Gaengel and Mlodzik, 2003)

NOT Enhancer of

Statement

Reference

pnut[+]/pnut^XP is a non-enhancer of mitotic domain 1 | embryonic cycle 14 phenotype of CycB^+t10

(Ji et al., 2002)

Suppressor of

Statement

Reference

pnut[+]/pnut^XP is a suppressor of retina phenotype of rdgB⁹

(Kumari et al., 2022)

pnut^XP is a suppressor of eye phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Kaltenbach et al., 2007)

pnut[+]/pnut^XP is a suppressor of eye phenotype of rpr^GMR.PW

(Gottfried et al., 2004)

pnut[+]/pnut^XP is a suppressor of eye phenotype of hid^GMR.PG

(Gottfried et al., 2004)

pnut[+]/pnut^XP is a suppressor of eye phenotype of grim^GMR.PC

(Gottfried et al., 2004)

NOT Suppressor of

Statement

Reference

pnut[+]/pnut^XP is a non-suppressor of mitotic domain 1 | embryonic cycle 14 phenotype of CycB^+t10

(Ji et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

pnut^XP/+ significantly suppresses the reduced eye phenotype caused by expression of grim^GMR.PC, W^GMR.PG or rpr^GMR.PW.

(Gottfried et al., 2004)

S^48-5/pnut^XP mutants show 24.58%+-5.90 misrotated ommatidia compared to 9.55%+-1.43 seen in S^48-5 mutants alone.

(Gaengel and Mlodzik, 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

pnut^mut1

(Akhmetova et al., 2018)

Rescued by

pnut^XP is rescued by pnut^{pnut.Tag:FLAG}

(Akhmetova et al., 2015)

pnut^XP is rescued by pnut^+t10

(Adam et al., 2000)

pnut^XP is rescued by pnut^+t10

(Neufeld and Rubin, 1994)

pnut^XP is rescued by pnut^hs.PN

(Neufeld and Rubin, 1994)

Partially rescued by

pnut^XP is partially rescued by pnut^{K288A.pnut.Tag:FLAG}

(Akhmetova et al., 2015)

pnut^XP is partially rescued by pnut^{1-427.pnut.Tag:FLAG}

(Akhmetova et al., 2015)

Not rescued by

pnut^XP is not rescued by pnut^{K155A.D206A.K288A.pnut.Tag:FLAG}

(Akhmetova et al., 2015)

Comments

pnut^XP fails to complement the lethality of pnut^mut1 mutants.

(Akhmetova et al., 2018)

pnut^{1-427.pnut.T:Zzzz\FLAG} fails to rescue the pnut^XP third-instar lethal phenotype. However, developing imaginal discs are found in such larvae, as compared with the disc-less phenotype of pnut^XP larvae.

pnut^XP mutant larvae also carrying pnut^{K155A.D206A.K288A.pnut.T:Zzzz\FLAG} show an indistinguishable phenotype from that of pnut^XP single mutants.

pnut^{K288A.pnut.T:Zzzz\FLAG} fails to rescue the lethality of pnut^XP. However, the cytokinesis defects associated with pnut^XP are rescued. Imaginal disc development is also rescued by pnut^{K288A.pnut.T:Zzzz\FLAG}.

(Akhmetova et al., 2015)

Lethality can be rescued by P{pnut} or P{hs-pnut}.

(Neufeld and Rubin, 1994)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5687

pnut^XP/T(2;3)SM6a-TM6B, Tb¹

Kyoto

108364

pnut^XP/T(2;3)SM6a-TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

(Kaltenbach et al., 2007)

dSEPT7 het

(Deb and Hasan, 2019)

dSEPT7 null

(Deb et al., 2016)

peanut-XP

(Gaengel and Mlodzik, 2003)

pnut^XP

(Dey et al., 2023, Kumari et al., 2022, Akhmetova et al., 2018, O'Neill and Clark, 2016, Akhmetova et al., 2015, Guillot and Lecuit, 2013, Christensen et al., 2008.12.28, Christensen et al., 2007.10.29)

pnut^xp

(Pinheiro et al., 2017, Deb et al., 2016, Adam et al., 2000)

pnutXP

(Gottfried et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (17)

Report Sections

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