FB2024_03 , released June 25, 2024
Allele: Dmel\NMGIIa.MtnA
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General Information
Symbol
Dmel\NMGIIa.MtnA
Species
D. melanogaster
Name
FlyBase ID
FBal0033833
Feature type
allele
Associated gene
Dmel\N
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct
(Periz and Fortini, 1999, Klueg et al., 1998, Frise et al., 1996, Matsuno et al., 1995, Rebay et al., 1991, Fehon et al., 1990, Smoller et al., 1990)
Progenitor genotype
Cytology
Description

Expression of full length N using the MtnA promoter.

(Matsuno et al., 1995)

Construct: N cDNA/genomic chimeric construct expression is driven by MtnA promoter.

(Fehon et al., 1990)

N is under the control of the MtnA promoter.

(Smoller et al., 1990)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      S2 cells expressing NMGIIa.MtnA can form heterotypic aggregates with S2 cells expressing DlMtnA.PF, DlΔELR1-3.MtnA, DlΔELR4-5.MtnA, DlΔICD.MtnA, DlC301Y.MtnA, DlC301S.MtnA, Dl::NrgNG2.MtnA or Dl::NrgNG3.MtnA.

      S2 cells expressing NMGIIa.MtnA cannot form heterotypic aggregates with S2 cells expressing DlΔaa32-198.MtnA, DlΔ192-331.MtnA, DlStu.MtnA, DlNae.MtnA, DlC288Y.MtnA, Dl::NrgNG1.MtnA or Dl::NrgNG4.MtnA.

      (Parks et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Carried in a plasmid, transfected into S2 cells, and used in assays to determine whether trans-endocytosis of Dl by N expressing cells is dependent on the Dl intracellular domain.

      (Klueg et al., 1998)

      Carried in plasmid "pRmHa-3-Notch" and expressed in Schneider cells as part of experiment to demonstrate physical interaction of N with Nle gene product in vivo.

      (Royet et al., 1998)

      Expressed in S2 cells.

      (Ray et al., 1999)

      Carried in plasmid "NotchWT", transfected into S2 cells and used in aggregation assays to assess the affinities of the homotypic and heterotypic interactions of Dl, Ser and N proteins and to examine whether fng protein regulates ligand-receptor binding.

      (Klueg and Muskavitch, 1999)

      Carried in plasmid "pMTNMg", cotransfected into S2 cells with a Psn expression construct and used to study N protein processing in these cells.

      (Ye and Fortini, 1999)

      Carried in plasmid "pMtNMg" and transfected into S2 cells to investigate the effects of Ca2+-ATPase inhibitors on N gene product localization.

      (Periz and Fortini, 1999)

      Carried in a plasmid and stably transfected into S2 cells.

      (Rand et al., 2000)

      Carried in a plasmid, transfected into S2 cells and EH34A3 cells (a shi1 cell line) and used to determine whether intercellular trafficking of N protein requires shi-dependent subcellular trafficking of Dl protein.

      (Parks et al., 2000)

      Carried in plasmid " pMT:NotchWT ", transfected into S2 cells and used in cell-cell aggregation assays.

      (Klueg et al., 2002)

      Transfected into S2 cells.

      (Bland et al., 2003)

      Carried in plasmid "pMTN" and transfected into S2 cells.

      (Sakata, 2004, Sakata et al., 2004)

      Carried in plasmid "pMTNMg" and transfected S2 cells for use in cell aggregation assays.

      (Parks et al., 2006)

      Carried in a plasmid and transfected into Schneider S2 cells.

      (Smoller et al., 1990)

      Carried in plasmid "pMtNMg" and transfected into S2 cells.

      (Rebay et al., 1991, Fehon et al., 1990)

      Carried in plasmid, used to transfect S2 cells.

      (Fortini and Artavanis-Tsakonas, 1994)

      Carried in plasmid "pMTNMg", expressed in S2 cells and used in a colocalisation assay of N and dx proteins.

      (Matsuno et al., 1995)

      Carried in a plasmid, expressed in Schneider S2 cells.

      (Bhanot et al., 1996)

      Carried in a plasmid, transfected into Schneider S2 cells, and used to study the ability of numb to inhibit Dl-dependent N activity.

      (Frise et al., 1996)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      N-S2
      (Rand et al., 2000)
      NMGIIa.MtnA
      Name Synonyms
      NotchWT
      (Parks et al., 2000)
      Secondary FlyBase IDs
        References (22)