NcoI-HindIII per cDNA fragment is fused to a NcoI-HindIII genomic fragment, expression is driven by an Hsp70 promoter.
Individuals are arrhythmic.
Some lines are rhythmic at 25oC or 29oC and others are not. Line 23a is arrhythmic and in a wild type strain per+ contributes to locomotor activity rhythms so they are indistinguishable from control wild type rhythms. Transient perturbations in per protein levels can phase-shift the circadian clock.