FlyBase Allele Report: Dmel\spz[2]

General Information

Symbol

Dmel\spz²

Species

D. melanogaster

Name

FlyBase ID

FBal0016060

Feature type

allele

Associated gene

Dmel\spz

Associated Insertion(s)

Carried in Construct

Also Known As

spz¹⁹⁷

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Lindsley and Zimm, 1992)

Mutagen

ethyl methanesulfonate

(Lindsley and Zimm, 1992, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Amino acid replacement: Y134N.

(Weber et al., 2007)

Nucleotide substitution: T400A.

(Weber et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:27,066,334..27,066,334 [-]

Nucleotide change:

T27066334A

Reported nucleotide change:

T400A

Amino acid change:

Reported amino acid change:

Y134N

(Weber et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm

(Zhu et al., 2008)

abnormal neuroanatomy

(Zhu et al., 2008)

partially lethal

(McIlroy et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

VA1d olfactory receptor neuron axon targeting is normal in mutant flies.

(Ward et al., 2015)

spz² mutant embryos exhibit aberrant targeting by the SNa motor axons.

spz² flies can barely walk.

(Zhu et al., 2008)

Hemolymph clots from spz² third instar larvae show normal melanization.

(Bidla et al., 2007)

Homozygous females produce dorsalised embryos.

(DeLotto and DeLotto, 1998)

Level of Drs induction of bacterially challenged mutants is lower than in wild type. Pattern of response of CecA1 and CecA2 parallels that of Drs. Dpt and Dro remain fully inducible and pattern of expression of AttA and Def in intermediate. Infection of spz⁴/spz² mutants with A.fumigatus causes 3% survival 3 days postinfection, infection with E.coli causes 84% survival 3 days postinfection.

(Lemaitre et al., 1996)

Injection of snk^Δne.T:ea into the central region of homozygous embryos causes a dorsalized phenotype.

(Smith and DeLotto, 1994)

Perivitelline fluid from Tl and dl donors was capable of restoring polarized gastrulation movements and cuticular elements.

(Stein and Nusslein-Volhard, 1992)

Embryos derived from spz²/spz³ females are weakly dorsalised at 18^oC. The mesoderm and ventral furrow are reduced in size, and mesoderm only develops in the posterior part of the embryo.

(Leptin and Grunewald, 1990)

Embryos derived from homozygous females are dorsalised.

(Carroll et al., 1987)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

spz5^e03444, spz² has abnormal neuroanatomy phenotype, enhanceable by NT1⁴¹

(Zhu et al., 2008)

spz² has abnormal neuroanatomy phenotype, enhanceable by NT1⁴¹/spz5^e03444

(Zhu et al., 2008)

spz² has increased cell death phenotype, enhanceable by Tl^r3/Df(3R)ro80b

(Zhu et al., 2008)

NT1⁴¹, spz² has abnormal neuroanatomy phenotype, enhanceable by spz5^e03444

(Zhu et al., 2008)

NOT Enhanced by

Statement

Reference

spz² has increased cell death phenotype, non-enhanceable by NT1⁴¹

(Zhu et al., 2008)

Suppressed by

Statement

Reference

spz² has partially lethal phenotype, suppressible by Scer\GAL4^elav-C155/Tl^10b.UAS.cYa

(McIlroy et al., 2013)

spz² has partially lethal phenotype, suppressible by Scer\GAL4^elav-C155/Toll-6^{C1020Y.UAS.Tag:FLAG}

(McIlroy et al., 2013)

spz² has partially lethal phenotype, suppressible by Toll-7^{C993Y.UAS.Tag:FLAG}/Scer\GAL4^elav-C155

(McIlroy et al., 2013)

Enhancer of

Statement

Reference

spz²/NT1⁴¹ is an enhancer of abnormal neuroanatomy phenotype of spz5^e03444

(Zhu et al., 2008)

spz²/spz5^e03444 is an enhancer of abnormal neuroanatomy phenotype of NT1⁴¹

(Zhu et al., 2008)

spz² is an enhancer of abnormal neuroanatomy phenotype of NT1⁴¹, spz5^e03444

(Zhu et al., 2008)

spz² is an enhancer of increased cell death phenotype of Df(3R)ro80b, Tl^10b.UAS

(Zhu et al., 2008)

NOT Enhancer of

Statement

Reference

spz² is a non-enhancer of abnormal neuroanatomy phenotype of NT1⁴¹

(Zhu et al., 2008)

spz² is a non-enhancer of increased cell death phenotype of NT1⁴¹

(Zhu et al., 2008)

spz² is a non-enhancer of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

NOT Suppressor of

Statement

Reference

spz² is a non-suppressor of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

spz² is a non-suppressor of partially lethal - majority die | recessive phenotype of cact⁷

(Qiu et al., 1998)

Other

Statement

Reference

NT1⁴¹, spz² has lethal phenotype

(Zhu et al., 2008)

NT1⁴¹, spz5^e03444, spz² has abnormal locomotor rhythm phenotype

(Zhu et al., 2008)

Df(3L)ED4342/NT1⁴¹, spz5^e03444, spz² has abnormal locomotor rhythm phenotype

(Zhu et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

spz5^e03444, spz² has larval intersegmental nerve branch ISNd of A1-7 phenotype, enhanceable by NT1⁴¹

(Zhu et al., 2008)

spz5^e03444, spz² has larval segmental nerve phenotype, enhanceable by NT1⁴¹

(Zhu et al., 2008)

spz5^e03444, spz² has larval transverse nerve phenotype, enhanceable by NT1⁴¹

(Zhu et al., 2008)

spz² has larval intersegmental nerve branch ISNb of A1-7 phenotype, enhanceable by NT1⁴¹/spz5^e03444

(Zhu et al., 2008)

spz² has larval intersegmental nerve branch ISNd of A1-7 phenotype, enhanceable by NT1⁴¹/spz5^e03444

(Zhu et al., 2008)

spz² has larval segmental nerve branch SNa of A1-7 phenotype, enhanceable by NT1⁴¹/spz5^e03444

(Zhu et al., 2008)

spz² has larval segmental nerve phenotype, enhanceable by NT1⁴¹/spz5^e03444

(Zhu et al., 2008)

spz² has larval transverse nerve phenotype, enhanceable by NT1⁴¹/spz5^e03444

(Zhu et al., 2008)

NT1⁴¹, spz² has larval intersegmental nerve branch ISNb of A1-7 phenotype, enhanceable by spz5^e03444

(Zhu et al., 2008)

NT1⁴¹, spz² has larval intersegmental nerve branch ISNd of A1-7 phenotype, enhanceable by spz5^e03444

(Zhu et al., 2008)

NT1⁴¹, spz² has larval segmental nerve phenotype, enhanceable by spz5^e03444

(Zhu et al., 2008)

NT1⁴¹, spz² has larval transverse nerve phenotype, enhanceable by spz5^e03444

(Zhu et al., 2008)

spz5^e03444, spz² has larval intersegmental nerve branch ISNb of A1-7 phenotype, enhanceable by NT1⁴¹

(Zhu et al., 2008)

Enhancer of

Statement

Reference

spz²/NT1⁴¹ is an enhancer of larval intersegmental nerve branch ISNb of A1-7 phenotype of spz5^e03444

(Zhu et al., 2008)

spz²/NT1⁴¹ is an enhancer of larval intersegmental nerve branch ISNd of A1-7 phenotype of spz5^e03444

(Zhu et al., 2008)

spz²/NT1⁴¹ is an enhancer of larval segmental nerve branch SNa of A1-7 phenotype of spz5^e03444

(Zhu et al., 2008)

spz²/NT1⁴¹ is an enhancer of larval segmental nerve phenotype of spz5^e03444

(Zhu et al., 2008)

spz²/NT1⁴¹ is an enhancer of larval transverse nerve phenotype of spz5^e03444

(Zhu et al., 2008)

spz²/spz5^e03444 is an enhancer of larval intersegmental nerve branch ISNb of A1-7 phenotype of NT1⁴¹

(Zhu et al., 2008)

spz²/spz5^e03444 is an enhancer of larval intersegmental nerve branch ISNd of A1-7 phenotype of NT1⁴¹

(Zhu et al., 2008)

spz²/spz5^e03444 is an enhancer of larval segmental nerve branch SNa of A1-7 phenotype of NT1⁴¹

(Zhu et al., 2008)

spz²/spz5^e03444 is an enhancer of larval segmental nerve phenotype of NT1⁴¹

(Zhu et al., 2008)

spz²/spz5^e03444 is an enhancer of larval transverse nerve phenotype of NT1⁴¹

(Zhu et al., 2008)

spz² is an enhancer of larval intersegmental nerve branch ISNb of A1-7 phenotype of NT1⁴¹, spz5^e03444

(Zhu et al., 2008)

spz² is an enhancer of larval intersegmental nerve branch ISNd of A1-7 phenotype of NT1⁴¹, spz5^e03444

(Zhu et al., 2008)

spz² is an enhancer of larval segmental nerve phenotype of NT1⁴¹, spz5^e03444

(Zhu et al., 2008)

spz² is an enhancer of larval transverse nerve phenotype of NT1⁴¹, spz5^e03444

(Zhu et al., 2008)

NOT Enhancer of

Statement

Reference

spz² is a non-enhancer of larval segmental nerve phenotype of NT1⁴¹

(Zhu et al., 2008)

spz² is a non-enhancer of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

NOT Suppressor of

Statement

Reference

spz² is a non-suppressor of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Pan-neuronal expression of Tl^{10b.Scer\UAS.cYa}, under the control of Scer\GAL4^elav-C155, suppresses the semi-lethality seen in spz² mutants.

Pan-neuronal expression of Toll-6^{C1020Y.Scer\UAS.T:Zzzz\FLAG}, under the control of Scer\GAL4^elav-C155, suppresses the semi-lethality seen in spz² mutants.

Pan-neuronal expression of Toll-7^{C993Y.Scer\UAS.T:Zzzz\FLAG}, under the control of Scer\GAL4^elav-C155, suppresses the semi-lethality seen in spz² mutants.

(McIlroy et al., 2013)

The SNa axonal phenotype of spz² NT1⁴¹ double-mutant embryos is epistatic to spz.

Levels of apoptosis increase in the CNS of spz² and Tl^r3/Df(3R)ro80b mutant embryos, indicating that both spz and Tl are required for neuronal survival.

Levels of apoptosis do not increase further in spz² NT1⁴¹ double mutants (compared to spz² mutants).

Whereas homozygous spz² flies can eclose as adults, the NT1⁴¹ spz² double mutants are completely lethal (100% penetrance).

The penetrance of both SNa and ISNb/d targeting defects increases in spz5^e03444 NT1⁴¹ spz², compared to the double or single mutants. In the triple mutants, misrouting phenotypes can be very dramatic, and there are cases of loss of all ISNb/d motor axons (not seen in single mutants). Misrouting of the transverse nerve (TN) can be very dramatic in triple mutants, although milder effects in this nerve occur with comparable penetrance in all genotypes (~10%). Misroutings of ISN are negligible in single and double mutants, but they increase and can be dramatic in triple-mutant embryos (12.7%).

NT1⁴¹ spz5^e03444 / NT1⁴¹ Df(3L)Exel6092 double mutant flies display a range of behavioral phenotypes including: inability to estimate the location of a petri dish rim, falling off a petri dish rim, sluggishness, inability to climb, and wobbling.

(Zhu et al., 2008)

Perivitelline fluid from embryos laid by Tl^rv13 spz⁴/Tl^rv4 spz² females does not show axis-inducing activity when injected into the perivitelline space of embryos derived from pip¹/pip² females. Perivitelline fluid from embryos laid by ea^D3 spz⁴/ea¹ spz² females does not show axis-inducing activity when injected into the perivitelline space of embryos derived from pip¹/pip² females. Perivitelline fluid from embryos laid by ea^D1 spz²/ea⁴ spz⁴ females shows axis-inducing activity when injected into the the perivitelline space of embryos derived from pip¹/pip² females.

(Chang and Morisato, 2002)

The zygotic semi-lethality of cact⁷ homozygotes is not suppressed by spz².

(Qiu et al., 1998)

Double mutant combinations with ea^D1 are strongly dorsalizing.

(Chasan et al., 1992)

Xenogenetic Interactions

Statement

Reference

The presence of one copy of spz² markedly reduces the number of flies with melanin deposits caused by expression of Hsap\CHMP2B^{Intron5.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

(Ahmad et al., 2009)

Complementation and Rescue Data

Comments

Microinjection of purified spz^2.1 protein into the perivitelline space of syncytial blastoderm stage embryos rescues embryonic phenotype: restores the ventrolateral pattern elements and normalises the dorsal-ventral axis of the recipient embryo.

(DeLotto and DeLotto, 1998)

Images (0)

Mutant

Wild-type

Stocks (2)

3115

106855

Notes on Origin