FB2024_03 , released June 25, 2024
Allele: Dmel\parats2
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General Information
Symbol
Dmel\parats2
Species
D. melanogaster
Name
FlyBase ID
FBal0013492
Feature type
allele
Associated gene
Dmel\para
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Pittendrigh et al., 1997)
Progenitor genotype
Cytology
Description

Mutation within the intracellular S4-S5 linker, in domain I.

(ffrench-Constant et al., 1998)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
X:16,506,220..16,506,220 [-]
Nucleotide change:

T16506220A

Amino acid change:

I265N | para-PA; I265N | para-PAA; I265N | para-PAB; I265N | para-PAC; I254N | para-PAD; I265N | para-PAE; I254N | para-PAF; I254N | para-PAG; I254N | para-PAH; I254N | para-PAI; I254N | para-PAJ; I254N | para-PAK; I254N | para-PAL; I254N | para-PAM; I265N | para-PAN; I265N | para-PAO; I265N | para-PAP; I265N | para-PAQ; H180Q | para-PAR; I211N | para-PAS; I211N | para-PAT; I211N | para-PAU; I211N | para-PAV; I254N | para-PAW; I254N | para-PAX; I254N | para-PAY; I254N | para-PAZ; I265N | para-PB; I254N | para-PBA; I254N | para-PBB; I254N | para-PBC; I254N | para-PBD; I254N | para-PBE; I265N | para-PBF; I265N | para-PBG; I253N | para-PBH; I265N | para-PC; I254N | para-PD; I265N | para-PE; I265N | para-PF; I265N | para-PG; I254N | para-PH; I265N | para-PI; I265N | para-PJ; I265N | para-PK; I265N | para-PL; I265N | para-PM; I265N | para-PN; I265N | para-PO; I265N | para-PP; I265N | para-PQ; I265N | para-PR; I265N | para-PS; I265N | para-PT; I265N | para-PU; I265N | para-PV; I265N | para-PW; I265N | para-PX; I265N | para-PY; I265N | para-PZ

Reported amino acid change:

I?N

Comment:

The nature of the nucleotide substitution in the mutant was inferred by a FlyBase curator based on the reported amino acid change, which is apparently the same as parats1. The location of the amino acid change was reported in the context of the surrounding amino acid sequence.

(Pittendrigh et al., 1997)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      parats2 mutant larvae display modest resistance to picrotoxin (PTX)-induced seizures.

      (Stilwell et al., 2006)

      Homozygous flies paralyse within 15 seconds at 38oC and recover as rapidly after shifting to 20oC. comt6 parats2 double homozygotes paralyse within 15 seconds at 38oC (a time course similar to parats2 single homozygotes). A burst of activity preceeding paralysis (as seen in comt6 single homozygotes) does not occur in the double mutants. After shifting to 20oC, the double mutant flies appear to recover immediately and after a period of 10 seconds into recovery, almost all the flies are upright. During this period, increased activity is observed, but by 30 seconds, more than half the upright flies paralyse again, and this paralysis is complete by 1 minute. Recovery after this second round of paralysis proceeds in a manner similar to that of comt6 single mutant flies, albeit somewhat faster. Spontaneous activity in the dorsal longitudinal muscles of parats2 single mutant flies is infrequent and usually undetectable at 20 or 36oC, as well as on cooling to 32oC. The burst of spontaneous activity seen at 36oC in the dorsal longitudinal muscles of comt6 single mutant flies is completely suppressed in comt6 parats2 double homozygotes. On cooling to 32oC, a robust comt6-like burst of spontaneous firing is seen in the double mutant flies (this is not seen in comt6 single mutant flies).

      (Sanyal et al., 1999)

      Mutant flies show an increased tolerance to the paralytic effect of the toxin AaIT compared to wild-type flies.

      (Zlotkin et al., 1999)

      Homozygous flies show significant resistance to dichloro-diphenyl-trichloro ethane (DDT) compared to wild-type flies.

      (Pittendrigh et al., 1997)

      Sub-anaesthetic concentration of carbon dioxide enhances the paralytic phenotype.

      (Krishnan et al., 1996)

      Marked reduction in number of embryonic neurons in cell culture expressing sodium currents in these alleles (O'Dowd, Germeraad and Aldrich, 1987; O'Dowd, Germeraad and Aldrich 1989). temperature-sensitive

      External Data
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      Phenotypic Class
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      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
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      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      para ts2
      (Zlotkin et al., 1999)
      parats2
      (Rikhy et al., 2007, Stilwell et al., 2006)
      Name Synonyms
      Secondary FlyBase IDs
        References (9)