FB2024_03 , released June 25, 2024
Allele: Dmel\mei-41D9
Open Close
General Information
Symbol
Dmel\mei-41D9
Species
D. melanogaster
Name
FlyBase ID
FBal0012160
Feature type
allele
Associated gene
Dmel\mei-41
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Mason et al., 1981)
Progenitor genotype
Cytology
Description

Amino acid replacement: S647F.

(Laurencon et al., 2003)

In addition to the S647F amino acid replacement, which may cause the mutant phenotype, a number of additional amino acid changes are present compared to the U34925 mei-41 GenBank sequence (these "common" mutations are also present in other mei-41 mutant alleles, possibly due to variability present in the mutagenised population).

(Laurencon et al., 2003)

Nucleotide substitution: C4257T.

(Laurencon et al., 2003)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
X:16,394,248..16,394,248 [+]
Nucleotide change:

C16394248T

Reported nucleotide change:

C4257T

Amino acid change:

S810F | mei-41-PA

Reported amino acid change:

S647F

Comment:

The mutation site reported in FBrf0160718 is relative to GB:U34925 (in which the predicted CDS is missing 163 N-terminal amino acids relative to genome release 3.2). Other amino acid changes common to several mei-41 mutants are also present in the strain (see FBrf0160718).

(Laurencon et al., 2003)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Mutant larvae show a largely normal checkpoint response (a steep decline in the number of G2 cells entering mitosis) in response to irradiation with 4000 rad of X rays, but they show an intermediate checkpoint phenotype when irradiated with 500 rad (showing only a small decline in the number of cells entering mitosis).

      (Bi et al., 2005)

      77% of eggs derived from homozygous females hatch into larvae. Hemizygous larvae show 70% loss of the G2/M checkpoint (this number is the average number of mitotic cells per eye disc after exposure of male hemizygous larvae to 500R of X rays expressed as a percentage of the number of mitotic cells per eye disc before irradiation, wild-type values range from 5 to 15%).

      (Laurencon et al., 2003)

      Hemizygous male larvae are sensitive to HN2 and MMS. Homozygous females are fertile and show no evidence of meiotic nondisjunction or chromosome loss.

      (Mason et al., 1981)

      Does not reduce meiotic exchange.

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      mei-41D9 is an enhancer of chromosome & neuroblast | third instar larval stage 2 phenotype of tefuunspecified

      (Bi et al., 2005)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Df(3R)d58/Df(3R)d189, P{CaSpeR-DTLum} (i.e. moi mutants) die at later phases or even hatch when they are also mutant for mei-41D9.

      (Komonyi et al., 2009)

      mei-41D9 ; tefustg double mutant larvae show complete loss of the checkpoint response when irradiated with either 500 or 4000 rad of X rays.

      (Bi et al., 2005)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      mei-41D9
      (Duan et al., 2020, Kurzhals et al., 2011, Komonyi et al., 2009, Bi et al., 2005)
      Name Synonyms
      Secondary FlyBase IDs
        References (10)