146bp deletion near coordinate -2.0 (the 0 coordinate marks the P-element insertion in mam45 - see FBrf0048222), in exon 4. This results in a frameshift, producing a predicted protein with 377 normal residues, followed by 69 amino acids from the frameshift, before a premature termination after residue 446.
No P-element detectable by in situ hybridisation. 100bp deletion.
Position of mutation on reference sequence inferred by FlyBase curator based on author statement: 146 bp deletion results in a frameshift, producing a predicted protein with 377 normal residues, followed by 69 amino acids from the frameshift, before a premature termination after residue 446.
Extreme phenotype. Larvae have cuticular abnormalities, including the absence of large portions of the cephalic and ventral epidermis. The anterior end of the embryo is filled with neural cells and the ventral nerve cord is expanded.
strong allele, P excised
P-element or transposase source: Harwich P strain.