A deletion of ~19 kb encompassing the entire halo gene.
Deletion of halo resulting from FLP-catalyzed recombination using the FRT-containing transposons PBac{WH}CG18131f04301 and PBac{WH}f07557.
Df(2L)ΔhaloAJ/Df(2L)ΔhaloAJ embryos lack inward lipid droplet transport during phase IIa (cycle 13 to mid-cellularization) of embryogenesis. Two copies of halot3.2 or halo3.2.T:Ivir\HA1 restores net inward droplet transport substantially but not completely. In wech7/wech7 Df(2L)ΔhaloAJ/Df(2L)ΔhaloAJ double mutant embryos, lipid droplets are peripheral. Increased halo dosage (halot3.2/halot3.2) in embryos results in an earlier inward shift and overall greater inward displacement of lipid droplets (comparing embryos with 0 - 4 copies of halo using combinations of halo3.2.T:Ivir\HA1 and Df(2L)ΔhaloAJ). Df(2L)ΔhaloAJ/Df(2L)ΔhaloAJ embryos with Lsd-2KG00149 knockdown display droplet accumulation below the nuclei, thus droplets are shifted outward (compared to position in embryos with Lsd-2KG00149 knockdown alone. KhcUbi-p63E.T:Hsap\MYC overexpression attenuates net motion of lipid droplets in the plus-end direction, with the droplet population shifting inwards as halo dosage increases (phase II embryos).
