A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
Df(3R)BSC547/Df(3R)BSC846 transheterozygous embryos do not show any obvious musculature phenotype.
Df(3R)BSC846 homozygous embryos show severe musculature defects.
The presence of P+PBac{XP5.WH5}BSC846 was verified using the PCR methods and primers described in FBrf0175003.
The breakpoints of Df(3R)BSC846 predicted from the Release 5 genomic coordinates of the insertion sites of the progenitor transposable elements PBac{WH}CG14509f04904 and P{XP}d00146 are 3R:25017393 ;25589319 and the cytological breakpoints predicted from these coordinates are 99A1;99B10.