[99B5-99B5];[99C2-99C2];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
99B5;99C2
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Both Df(3R)BSC547/Df(3R)01215 and Df(3R)BSC547/Df(3R)BSC846 transheterozygous embryos do not show any obvious musculature phenotype.
In Df(3R)BSC547 homozygous embryos show variable phenotypes, which may include: germ-band retraction and/or dorsal closure fail, but the musculature is mostly present; pharynx musculature is present, but most other musculature is missing; nothing besides the dorsoventral axis is recognizable.
Df(3R)BSC547 heterozygous adults previously entrained under 12h:12h light:dark cycles exhibit a significant decrease in the proportion of individuals exhibiting circadian rhythmicity upon shift to constant darkness conditions, despite exhibiting an apparently normal circadian period, as compared to controls.
The presence of P+PBac{XP5.WH5}BSC547 was verified using the PCR methods and primers described in FBrf0175003.
The cytological breakpoints of Df(3R)BSC547 predicted from the Release 5 genomic coordinates of the progenitor PBac{WH}f01048 and P{XP}CG7802d07781 transposable element insertion sites are 99B5;99C2.