[12E3-12E3];[12E7-12E7];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
12E3;12E7
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
1: P{XP}d10478
2: PBac{RB}nae04385
The presence of P+PBac{XP5.RB3}BSC628 was verified using the PCR methods and primers described in FBrf0175003, with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods.
The cytological breakpoints of Df(1)BSC628 predicted from the Release 5 genomic coordinates of the insertions sites of the progenitor P{XP}d10478 and PBac{RB}nae04385 transposable elements are 12E3;12E7.