[66D12-66D12];[67B3-67B3];
A set of isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Initial core set of 209 isogenic deletions provides ~60% euchromatic genome coverage.
66D12;67B3
This deletion removes a single ribosomal protein-coding gene (though other genes are also removed).
Df(3L)knittrigΔ1 embryos transheterozygous with Df(3L)ED4421 show reduced formation of ganglionic tracheal branches with significant penetrance - the majority of embryos show disruption in at least one ganglionic branch. No abnormal phenotype is detected for midline glial cells.
Df(3L)knittrigΔ1 flies transheterozygous with Df(3L)ED4421 show reduced viability and only rare escaper flies are found. Most mutants die during pupal stages or as pharate adults. Most escapers are unable to undergo complete wing inflation, causing wrinkled wings. Some escapers show partially inflated and abnormally opaque wings. Mutant pupal wings appar normal.
Df(3L)ED4421 results in cardiac enlargement in adults, but no significant reduction in fractional shortening, as compared to controls.
Flies heterozygous for the deletion do not show a Minute bristle phenotype.
Confirmed by 3-step PCR confirmation as shown in http://www.drosdel.org.uk/del_confirm.php Confirmed by lack of complementation to a known lethal mutation predicted to fall between the progenitor insertions.
Limits of break 1 computationally determined from location of progenitor P insertion on genome sequence between P{PZ}SrpRβrK561&P{lacW}l(3)j5B6j5B6 and P{PZ}l(3)0162901629&P{PZ}mRpL1210534 Limits of break 2 computationally determined from location of progenitor P insertion on genome sequence between P{EP}Hsp26EP3336&P{EP}Hsp26EP3315 and P{PZ}fry02240