FB2024_04 , released June 25, 2024
Recombinant Construct: P{UAS-lacZ.RNAi.K}
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General Information
Symbol
P{UAS-lacZ.RNAi.K}
FlyBase ID
FBtp0016505
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZRNAi.cKa.UAS
Also Known As
UAS-IRlacZ, LacZ IR, UAS-LacZ IR
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mC
Component allele Ecol\lacZRNAi.cKa.UAS
Transgenic product class
RNAi_reagent
(Kennerdell and Carthew, 2000)
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Ecol\lacZ
Description

UAS regulatory sequences drive expression of an inverted repeat of Ecol\lacZ coding sequences (nucleotides 3090-3835 relative to GenBank accession J01636). One copy of the repeat used to generate the construct had an EcoRI site at the nucleotide 3090 end and a SfiI site (top strand sequence GGCCATCTTGGCC) at the nucleotide 3835 end. The second copy of the repeat used had a XhoI site at the nucleotide 3090 end and a different SfiI site (top strand sequence GGCCTAGATGGCC) at the nucleotide 3835 end. The resulting SfiI site created in the construct has a central ATCTA sequence that is nonpalindromic. Consequently, the inverted repeat has imperfect dyad symmetry.

(Kennerdell and Carthew, 2000)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (11)
Reported As
Symbol Synonym
LacZ IR
(Ishida et al., 2012, Kuranaga et al., 2006)
LacZRNAi
(Samaraweera et al., 2013)
P{UAS-lacZ.IR}
(FlyBase, 1996-)
P{UAS-lacZ.RNAi.K}
(FlyBase, 1996-)
UAS-IRlacZ
(Presente et al., 2002, Kennerdell and Carthew, 2000)
UAS-LacZ IR
(Ishida et al., 2012, Lee et al., 2009)
UAS-LacZ-IR
(Sekine et al., 2011)
UAS-LacZIR
(Tonoki et al., 2009)
UAS-LacZi
(Presente et al., 2004)
UAS-lacZ IR
(Kanuka et al., 2005)
UASt-IRlacZ
(Pelisson et al., 2007)
Secondary FlyBase IDs
    References (14)