FB2024_04 , released June 25, 2024
Allele: Ecol\lacZRNAi.cKa.UAS
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General Information
Symbol
Ecol\lacZRNAi.cKa.UAS
Species
E. coli
Name
FlyBase ID
FBal0196940
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
Carried in Construct
P{UAS-lacZ.RNAi.K}
Also Known As
UAS-IRlacZ, LacZ IR, UAS-LacZ IR
Transgenic product class
RNAi_reagent
(Kennerdell and Carthew, 2000)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Kennerdell and Carthew, 2000)
Mutagen
in vitro construct
(Presente et al., 2004, Presente et al., 2002, Kennerdell and Carthew, 2000)
Progenitor genotype
Carried in construct
P{UAS-lacZ.RNAi.K}
Cytology
Description

UAS regulatory sequences drive expression of an inverted repeat of Ecol\lacZ coding sequences (nucleotides 3090-3835 relative to GenBank accession J01636). One copy of the repeat used to generate the construct had an EcoRI site at the nucleotide 3090 end and a SfiI site (top strand sequence GGCCATCTTGGCC) at the nucleotide 3835 end. The second copy of the repeat used had a XhoI site at the nucleotide 3090 end and a different SfiI site (top strand sequence GGCCTAGATGGCC) at the nucleotide 3835 end. The resulting SfiI site created in the construct has a central ATCTA sequence that is nonpalindromic. Consequently, the inverted repeat has imperfect dyad symmetry.

(Kennerdell and Carthew, 2000)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Ecol\lacZ
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (6)
      Reported As
      Symbol Synonym
      Ecol\lacZIR.dsRNA
      Ecol\lacZRNAi.IR.UAS
      Ecol\lacZRNAi.cKa.UAS
      Ecol\lacZdsRNA.IR.Scer\UAS
      Ecol\lacZdsRNA.IR.UAS
      Ecol\lacZdsRNA.Scer\UAS
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0141818
      • FBal0156541
      References (13)