TurboID corresponds to a promiscuous biotin ligase derived by mutation of the Escherichia coli birA gene (EcoGene:EG101230); TurboID contains the following amino acid substitutions relative to the wild-type protein; Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V160A, T192A, K194I, M209V, M241T, S263P and I305V (FBrf0240093). TurboID can thus be used as a proximity labeling tool for proteomic analysis; it has faster labeling kinetics than BirA(R118G); 10 minute labeling using TurboID produces similar proteome labeling as seen for 18 hour labeling using BirA(R118G) (FBrf0240093). The exact sequence of the TurboID biotin ligase may vary depending on the particular transgene or modified endogenous locus being used; the coding sequence may have been codon optimized for use in different species, and amino acid residue A146 may have been deleted to suppress dimerization. For a comparison of TurboID with other protein proximity labeling tools, see PMID:30774936, FBrf0240093.