FlyBase Experimental Tool Report: Tag:Mito(hCOX8A)

FB2024_04 , released June 25, 2024

FB2024_04 , released June 25, 2024

Experimental tool: Tag:Mito(hCOX8A)

General Information

Symbol

Tag:Mito(hCOX8A)

FlyBase ID

FBto0000224

Name

Mitochondrial targeting signal of COX8A

Description

Description

Mitochondrial targeting signal corresponding to the first 36 amino acids of the Homo sapiens COX8A gene (HGNC:2294). This peptide sequence can be used as a targeting signal to the mitochondrial matrix.

Uses

mitochondrial protein localization tag

External Crossreferences and Linkouts

Related experimental tools

Transgenic Constructs

Tagged with the tool (41)

Transgenic construct(s)

Component allele

Reg. region

Encoded product / tool

Tagged with

Also carries

Stocks

P{UAS-mito-HA-GFP.AP}

Avic\GFP^{S65T.UAS.Tag:HA,Tag:Mito(hCOX8A)}

UAS

GFP(S65T): green fluorescent protein

Tag:HA: epitope tag
Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-DsRed.mito}

Disc\RFP^{UAS.Tag:Mito(hCOX8A)}

UAS

DsRed(Unk): red fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{sqh-EYFP-Mito}

Avic\GFP^{EYFP.sqh.Tag:Mito(hCOX8A)}

sqh

EYFP: yellow fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{tub-mito-roGFP2-Orp1}

Avic\GFP^{roGFP2-Orp1.mito.αTub84B}

αTub84B

roGFP2-Orp1: small molecule sensor
fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-mito-APEX.FLAG}

Psat\APX1^{pAPEX.UAS.mito.Tag:FLAG}

UAS

pAPEX: reporter enzyme
proximity labeling tool (protein)

Tag:FLAG: epitope tag
Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-mito-PyronicSF}

Ecol\pdhR^{PyronicSF.UAS.Tag:Mito(hCOX8A)}

UASt

PyronicSF: fluorescent protein
small molecule sensor

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-mito-roGFP2-Grx1}

Avic\GFP^{roGFP2-Grx1.mito.UAS}

UASt

roGFP2-Grx1: redox state sensor
fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{tub-mito-roGFP2-Grx1}

Avic\GFP^{roGFP2-Grx1.mito.αTub84B}

αTub84B

roGFP2-Grx1: redox state sensor
fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-2mt8-TN-XXL}

Avic\GFP^{TN-XXL.UAS.Tag:Mito(hCOX8A)}

UAS

TN-XXL: calcium ion sensor
fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-mito-APEX.GFP}

Psat\APX1^{pAPEX.UAS.mito.GFP}

UAS

pAPEX: reporter enzyme
proximity labeling tool (protein)

GFP: green fluorescent protein
Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-mito-roGFP2-Orp1}

Avic\GFP^{roGFP2-Orp1.mito.UAS}

UASt

roGFP2-Orp1: small molecule sensor
fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-MitoTimer}

Disc\RFP^{MitoTimer.UAS}

UAS

DsRed(E5): fluorescent timer protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UASp-mito-GFP}

Avic\GFP^{E.UASp.Tag:Mito(hCOX8A)}

UASp

EGFP: green fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{13xlexAop2-APEX.Mito.HA}

Psat\APX1^{pAPEX.lexAop.Tag:HA,Tag:Mito(hCOX8A)}

lexAop

pAPEX: reporter enzyme
proximity labeling tool (protein)

Tag:HA: epitope tag
Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{dj-DsRed.MTS}

Disc\RFP^{DsRed2.dj.Tag:Mito(hCOX8A)}

dj

DsRed2: red fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{dj-Venus.MTS}

Avic\GFP^{Venus.dj.Tag:Mito(hCOX8A)}

dj

Venus: yellow fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{nanos-mito-roGFP2-Grx1.nanos3'UTR}

Avic\GFP^{roGFP2-Grx1.mito.nanos}

nanos

roGFP2-Grx1: redox state sensor
fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{nanosP-mito-roGFP2-Orp1-nanos3'UTR}

Avic\GFP^{roGFP2-Orp1.mito.nanos}

nanos

roGFP2-Orp1: small molecule sensor
fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{tub-mito-GFP}

Avic\GFP^{βTub56D.Tag:Mito(hCOX8A)}

βTub56D

GFP: green fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-2mt8-D4cpv}

Avic\GFP^{D4cpv.UAS.Tag:Mito(hCOX8A)}

UAS

Cameleon(D4cpv): calcium ion sensor
fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-4mt-GCaMP3}

Avic\GFP^{GCaMP3.UAS.Tag:Mito(hCOX8A)}

UAS

GCaMP3: calcium ion sensor
fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-aeq.mt}

Avic\aeq^{UAS.Tag:Mito(hCOX8A)}

UASt

aeq: bioluminescence reporter
calcium ion sensor

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-camgaroo-2.mt}

Avic\GFP^{camgaroo2.UAS.Tag:Mito(hCOX8A)}

UASt

camgaroo2: calcium ion sensor
fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-Dendra2.mito}

DspSSAL-2002\GFP^{Dendra2.UAS.Tag:Mito(hCOX8A)}

UAS

Dendra2: photoconvertible fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-mito-GFP.H}

Avic\GFP^{UAS.Tag:Mito(hCOX8A)}

UAS

GFP: green fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-mito-mKate}

Equa\eqFP578^{mKate.UAS.Tag:Mito(hCOX8A)}

UASt

mKate: far-red fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-MitoTimer.SD}

Disc\RFP^{MitoTimer.UAS.cSDa}

UASt

DsRed(E5): fluorescent timer protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-mitycam}

Avic\GFP^mitycam.UAS

UASt

Inverse-Pericam(T148.Q67): calcium ion sensor
fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-mtAlpHi}

Avic\GFP^mtAlpHi.UAS

UAS

AlpHi: pH sensor
fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-mt-mKeima.K}

Mosp\GFPL-20^{mKeima.UAS.cKa.Tag:Mito(hCOX8A)}

UASt

mKeima: pH sensor
red fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-mt-mKeima}

Mosp\GFPL-20^{mKeima.UAS.Tag:Mito(hCOX8A)}

UASt

mKeima: pH sensor
red fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UASp-mit.dendra2}

DspSSAL-2002\GFP^{Dendra2.UASp.Tag:Mito(hCOX8A)}

UASp

Dendra2: photoconvertible fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UASp-mito-KillerRed}

AspSL-2003\anm2CP^{KillerRed.UASp.Tag:Mito(hCOX8A)}

UASp

KillerRed: chromophore-assisted light inactivation tag
red fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UASp-Mito-moxMaple3}

Clsp\FP484^{moxMaple3.UASp.Tag:Mito(hCOX8A)}

UASp

moxMaple3: photoconvertible fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UASp-mito-PA-GFP}

Avic\GFP^{PA.UASp.Tag:Mito(hCOX8A)}

UASp

PA-GFP: green fluorescent protein
photoactivatable fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UASp-mito-SuperNova-Red}

AspSL-2003\anm2CP^{SuperNova-Red.UASp.Tag:Mito(hCOX8A)}

UASp

SuperNova-Red: chromophore-assisted light inactivation tag
red fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UASp-mKate2.mit}

Equa\eqFP578^{mKate2.UAS.Tag:Mito(hCOX8A)}

UASp

mKate2: red fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-Ratiometric-Pericam.mt}

Avic\GFP^{RatiometricPericam.UAS.Tag:Mito(hCOX8A)}

UASt

Ratiometric-Pericam: calcium ion sensor
green fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-tdTomato.mito}

Disc\RFP^{tdTomato.UAS.Tag:Mito(hCOX8A)}

UASt

tdTomato: red fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-YC2.0.mt}

Avic\GFP^{YC2.0.UAS.Tag:Mito(hCOX8A)}

UAS

Cameleon(YC2.0): calcium ion sensor
fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

PBac{UAS-mt-mKeima.C}

Mosp\GFPL-20^{mKeima.UAS.cCa.Tag:Mito(hCOX8A)}

UASt

mKeima: pH sensor
red fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

Insertions ( 3 )

Insertion

Encoded product / tool

Tagged with

Also carries

Stocks

Associated Alleles

P{UAS-matrix-QC}attP2

GFP: green fluorescent protein
mCherry: red fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-matrix-QC}attP16

GFP: green fluorescent protein
mCherry: red fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

P{UAS-matrix-QC}attP40

GFP: green fluorescent protein
mCherry: red fluorescent protein

Tag:Mito(hCOX8A): mitochondrial protein localization tag

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

T:Hsap\COX8A

(FlyBase, 1992-)

T:Hsap\Mito-COX8A

(FlyBase, 1992-)

T:Hsap\mito

(FlyBase, 1992-)

Tag:Mito(hCOX8A)

Name Synonyms

Mitochondrial targeting signal of COX8A

Secondary FlyBase IDs

FBgn0280211
FBgn0067048
FBgn0265462

References (35)

Report Sections