Hu, D., Smith, E.R., Garruss, A.S., Mohaghegh, N., Varberg, J.M., Lin, C., Jackson, J., Gao, X., Saraf, A., Florens, L., Washburn, M.P., Eissenberg, J.C., Shilatifard, A. (2013). The Little Elongation Complex Functions at Initiation and Elongation Phases of snRNA Gene Transcription.  Mol. Cell 51(4): 493--505.

FBrf0222548

Research paper

The small nuclear RNA (snRNA) genes have been widely used as a model system for understanding transcriptional regulation due to the unique aspects of their promoter structure, selectivity for either RNA polymerase (Pol) II or III, and because of their unique mechanism of termination that is tightly linked with the promoter. Recently, we identified the little elongation complex (LEC) in Drosophila that is required for the expression of Pol II-transcribed snRNA genes. Here, using Drosophila and mammalian systems, we provide genetic and molecular evidence that LEC functions in at least two phases of snRNA transcription: an initiation step requiring the ICE1 subunit, and an elongation step requiring ELL.

PMC4104523 (PMC) (EuropePMC)