Isolation and characterization of Df(2L)BSC827
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC827 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f04844 and P{XP}d05552. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of P{hsFLP}1, y1 w1118; PBac{WH}f04844/P{XP}d05552 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP3.WH3}BSC827 from the segment of PBac{WH}f04844 to the left of its FRT site and the segment of P{XP}d05552 to the right of its FRT site. The insertion site of PBac{WH}f04844 is Release 5 genomic coordinate 10260017--10260116 on arm 2L, which corresponds to 31B1. Exelixis, Inc. determined the insertion site of P{XP}d05552 to be Release 3 genomic coordinate 10314218 on chromosome arm 2L. This corresponds to  2L:10321809  and 31D7 on the Release 5 genome map. Consequently, the breakpoints of Df(2L)BSC827 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  2L:10260017--10260116 ;10321809 and the cytological breakpoints predicted from these coordinates are 31B1;31D7. Df(2L)BSC827 failed to complement trk1 and nmd08774.
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Kevin Cook, Ph.D
Bloomington Drosophila Stock Center
Department of Biology
Indiana University
1001 E. Third St.
Bloomington, IN 47405-7005
kercook@XXXX
812-856-1213 (office), 812-855-2577 (fax)
http://flystocks.bio.indiana.edu