From: 	Kevin Cook <kcook@XXXX>
To: 	FlyBase-Cambridge <flybase-cambridgeXXXX>, Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX>, kaufmanXXXX
Subject: 	Isolation and characterization of Df(2R)BSC603
Date: 	Wed, 03 Sep 2008  12:00:27  -0400  ( 17:00  BST)
Isolation and characterization of Df(2R)BSC603
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC603 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}bs[d01376] and PBac{WH}CG13579[f00432]. The 
deletion was isolated as a chromosome lacking miniwhite markers in 
progeny of P{hsFLP}1, y[1] w[1118]; 
P{XP}bs[d01376]/PBac{WH}CG13579[f00432] males crossed to w[1118]; 
P{hs-hid}2, wg[Sp-1]/SM6a females. These males were heat shocked as 
larvae as described in Parks et al., Nature Genetics 36: 288-292, 
2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC603 from the segment of 
P{XP}bs[d01376] to the left of its FRT site and the segment of 
PBac{WH}CG13579[f00432] to the right of its FRT site. Its presence 
was verified using the PCR methods and primers described in Parks et 
al. The cytological breakpoints of Df(2R)BSC603 predicted from the 
Release 5 genomic coordinates of the transposable element insertions 
sites are 60C7;60D1. Df(2R)BSC603 failed to complement Df(2R)ED4065 
and Mov34[k08003].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX