Date: Tue, 08 May 2007  08:46:01  -0400
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(3L)BSC282
Cc: mdealXXXX, Stacey Christensen <sjchristXXXX>, kaufman@XXXX
Isolation and characterization of Df(3L)BSC282
Stacey Christensen, Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC282 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d08188 and PBac{WH}dpr6[f07173]. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; P{XP}d08188/PBac{WH}dpr6[f07173] males. The males were heat 
shocked as larvae as described in Parks et al. Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC282 from the segment of P{XP}d08188 
to the left of its FRT site and the segment of PBac{WH}dpr6[f07173] 
to the right of its FRT site. Its presence was verified using the PCR 
methods and primers described in Parks et al. The cytological 
breakpoints of Df(3L)BSC282 predicted from the Release 5 genomic 
coordinates of the transposable element insertions sites are 
67C7;67D5. Df(3L)BSC282 failed to complement CG8108[EY14316] and Df(3L)AC1.
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX